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AB16301 Anti-Nitric Oxide Synthase III Antibody

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AB16301
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB16301
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase III Antibody
      Alternate Names
      • NOS III
      • eNOS
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nitric Oxide Synthase III Antibody detects level of Nitric Oxide Synthase III & has been published & validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot: 5 μg/mL using chemiluminescent detection. Detects ~135-140kDa band in endothelial cell preparations; Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 5-10 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 596-610 of human endothelial NOS, coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReactive with human NOS-III (eNOS). This immunogen sequence is 100% homologous in human, bovine, rat, and mouse, but reactivity has not been tested.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS3
      • eNOS
      • cNOS
      • ECNOS
      • EC-NOS
      • NOSIII
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1203 amino acids; 133289 Da
      SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.
      SUBCELLULAR LOCATION: Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
      TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.
      SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase III Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase III Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NITRIC OXIDE SYNTHASE III (NOS-III; eNOS) 2488941
      RABBIT ANTI-NITRIC OXIDE SYNTHASE III (NOS-III; eNOS) - 3514197 3514197
      RABBIT ANTI-NITRIC OXIDE SYNTHASE III (NOS-III; eNOS) -2780430 2780430
      RABBIT ANTI-NITRIC OXIDE SYNTHASE III (NOS-III; eNOS) -2844440 2844440

      References

      Reference overviewPub Med ID
      Angiotensin II-induced contraction is attenuated by nitric oxide in afferent arterioles from the nonclipped kidney in 2K1C.
      Helle, F; Hultström, M; Skogstrand, T; Palm, F; Iversen, BM
      American journal of physiology. Renal physiology  296  F78-86  2009

      Show Abstract
      18945823 18945823
      The interactive effects of hypoxia and nitric oxide on catecholamine secretion in rainbow trout (Oncorhynchus mykiss).
      McNeill, B; Perry, SF
      The Journal of experimental biology  209  4214-23  2006

      Show Abstract
      17050836 17050836
      Morphological support for paracrine inhibition of gastric acid secretion by nitric oxide in humans
      Berg, A, et al
      Scand J Gastroenterol, 36:1016-21 (2001)  2001

      11589372 11589372

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Title
      RABBIT ANTI-NITRIC OXIDE SYNTHASE III (NOS-III; eNOS)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies