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AB5384 Anti-Nitric Oxide Synthase II Antibody

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AB5384
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5384
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase II Antibody
      Alternate Names
      • NOS II
      • iNOS
      Background InformationThe inducible isoform of the enzyme nitric oxide synthase (iNOS, NOS-II) is synthesized by macrophages and many other cell types in response to proinflammatory cytokines and certain other stimuli, such as hypoxia and stress.
      References
      Product Information
      FormatSerum
      PresentationRabbit serum. Liquid containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nitric Oxide Synthase II using this Anti-Nitric Oxide Synthase II Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot: 1:1,000-1:2,500 (ECL).

      Immunohistochemistry: 1:2,500-1:6,000 (ABC).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenC-terminal 19 amino acid peptide from human chondrocyte nitric oxide synthase.
      HostRabbit
      SpecificityInducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with nNOS and eNOS by immunohistochemistry. AB5384 reacts with human glia and certain neurons in Alzheimer's disease by immunohistochemistry.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
      Gene Symbol
      • NOS2A
      • iNOS
      • HEP-NOS
      • INOS
      • NOS
      • NOS2
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
      COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
      SIZE: 1153 amino acids; 131117 Da
      SUBUNIT: Homodimer. Binds SLC9A3R1.
      TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
      SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase II Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase II Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) 2470342
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) - 3722145 3722145
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) POLYCLONAL ANTIBODY 3143910
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) POLYCLONAL ANTIBODY 3040524
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) POLYCLONAL ANTIBODY 2884790
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS) POLYCLONAL ANTIBODY -2506316 2506316

      References

      Reference overviewPub Med ID
      Imaging pulmonary inducible nitric oxide synthase expression with PET.
      Huang, HJ; Isakow, W; Byers, DE; Engle, JT; Griffin, EA; Kemp, D; Brody, SL; Gropler, RJ; Miller, JP; Chu, W; Zhou, D; Pierce, RA; Castro, M; Mach, RH; Chen, DL
      Journal of nuclear medicine : official publication, Society of Nuclear Medicine  56  76-81  2015

      Show Abstract
      25525182 25525182
      Oxidative tissue injury in multiple sclerosis is only partly reflected in experimental disease models.
      Schuh, C; Wimmer, I; Hametner, S; Haider, L; Van Dam, AM; Liblau, RS; Smith, KJ; Probert, L; Binder, CJ; Bauer, J; Bradl, M; Mahad, D; Lassmann, H
      Acta neuropathologica  128  247-66  2014

      Show Abstract
      24622774 24622774
      Multiple sclerosis deep grey matter: the relation between demyelination, neurodegeneration, inflammation and iron.
      Haider, L; Simeonidou, C; Steinberger, G; Hametner, S; Grigoriadis, N; Deretzi, G; Kovacs, GG; Kutzelnigg, A; Lassmann, H; Frischer, JM
      Journal of neurology, neurosurgery, and psychiatry  85  1386-95  2014

      Show Abstract
      24899728 24899728
      Disease-specific molecular events in cortical multiple sclerosis lesions.
      Fischer, MT; Wimmer, I; Höftberger, R; Gerlach, S; Haider, L; Zrzavy, T; Hametner, S; Mahad, D; Binder, CJ; Krumbholz, M; Bauer, J; Bradl, M; Lassmann, H
      Brain : a journal of neurology  136  1799-815  2013

      Show Abstract
      23687122 23687122
      Nitric oxide stress in sporadic inclusion body myositis muscle fibres: inhibition of inducible nitric oxide synthase prevents interleukin-1β-induced accumulation of β-amyloid and cell death.
      Schmidt, J; Barthel, K; Zschüntzsch, J; Muth, IE; Swindle, EJ; Hombach, A; Sehmisch, S; Wrede, A; Lühder, F; Gold, R; Dalakas, MC
      Brain : a journal of neurology  135  1102-14  2012

      Show Abstract
      22436237 22436237
      Protein co-expression with axonal injury in multiple sclerosis plaques.
      Maria Diaz-Sanchez, Kelly Williams, Gabriele C DeLuca, Margaret M Esiri
      Acta neuropathologica  111  289-99  2006

      Show Abstract
      16547760 16547760
      Effect of IBD sera on expression of inducible and endothelial nitric oxide synthase in human umbilical vein endothelial cells.
      Palatka, K; Serfozo, Z; Veréb, Z; Bátori, R; Lontay, B; Hargitay, Z; Nemes, Z; Udvardy, M; Erdodi, F; Altorjay, I
      World journal of gastroenterology  12  1730-8  2006

      Show Abstract
      16586542 16586542

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Title
      RABBIT ANTI-INDUCIBLE NITRIC OXIDE SYNTHASE (iNOS)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies