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AB5382 Anti-Nitric Oxide Synthase II Antibody

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IHC, WBRbSerumPolyclonal Antibody
      Catalogue NumberAB5382
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase II Antibody
      Alternate Names
      • NOS II
      • iNOS
      Background InformationNitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.
      Product Information
      • Macrophages, liver lysates
      PresentationRabbit polyclonal serum in buffer containing liquid 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).

      1:50,000 dilution of a previous lot was used on cultured cells.

      Western blot:
      1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenC-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityInducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse and rat. Reactivity to human iNOS is approximately 1%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
      Gene Symbol
      • NOS2A
      • iNOS
      • HEP-NOS
      • INOS
      • NOS
      • NOS2
      • EC
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
      COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
      SIZE: 1153 amino acids; 131117 Da
      SUBUNIT: Homodimer. Binds SLC9A3R1.
      TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
      SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Molecular Weight131 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Mouse liver lysates.

      Western Blot Analysis:
      1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at -20°C in undiluted aliquots from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information


      Anti-Nitric Oxide Synthase II Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase II Antibody Certificates of Analysis

      TitleLot Number
      Anti-Nitric Oxide Synthase II 2459002
      Anti-Nitric Oxide Synthase II - 2392230 2392230
      Anti-Nitric Oxide Synthase II - 2064696 2064696
      Anti-Nitric Oxide Synthase II - 2227120 2227120
      Anti-Nitric Oxide Synthase II - 3217778 3217778
      Anti-Nitric Oxide Synthase II - 3283233 3283233
      Anti-Nitric Oxide Synthase II - 3425718 3425718
      Anti-Nitric Oxide Synthase II - 3597326 3597326
      Anti-Nitric Oxide Synthase II - 3728343 3728343
      Anti-Nitric Oxide Synthase II - JBC1766784 JBC1766784


      Reference overviewApplicationSpeciesPub Med ID
      Mitral and tufted cells are potential cellular targets of nitration in the olfactory bulb of aged mice.
      Yang, MJ; Sim, S; Jeon, JH; Jeong, E; Kim, HC; Park, YJ; Kim, IB
      PloS one  8  e59673  2013

      Show Abstract
      Immunofluorescence23527248 23527248
      Dry needling at myofascial trigger spots of rabbit skeletal muscles modulates the biochemicals associated with pain, inflammation, and hypoxia.
      Hsieh, YL; Yang, SA; Yang, CC; Chou, LW
      Evidence-based complementary and alternative medicine : eCAM  2012  342165  2012

      Show Abstract
      Western BlottingRabbit23346198 23346198
      Spontaneously hypertensive rats display reduced microglial activation in response to ischemic stroke and lipopolysaccharide.
      De Geyter, D; Stoop, W; Zgavc, T; Sarre, S; Michotte, Y; De Keyser, J; Kooijman, R
      Journal of neuroinflammation  9  114  2012

      Show Abstract
      22647642 22647642
      Proteomic characterization of the cellular response to nitrosative stress mediated by s-nitrosoglutathione reductase inhibition.
      Foster, MW; Yang, Z; Gooden, DM; Thompson, JW; Ball, CH; Turner, ME; Hou, Y; Pi, J; Moseley, MA; Que, LG
      Journal of proteome research  11  2480-91  2012

      Show Abstract
      22390303 22390303
      Localization of a wide-ranging panel of antigens in the rat retina by immunohistochemistry: comparison of Davidson's solution and formalin as fixatives.
      Chidlow, G; Daymon, M; Wood, JP; Casson, RJ
      The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society  59  884-98  2011

      Show Abstract
      21832149 21832149
      Dexamethasone inhibits the Nox-dependent ROS production via suppression of MKP-1-dependent MAPK pathways in activated microglia.
      Huo, Y; Rangarajan, P; Ling, EA; Dheen, ST
      BMC neuroscience  12  49  2011

      Show Abstract Full Text Article
      21615929 21615929
      Peripheral purinergic receptor modulation influences the trigeminal ganglia nitroxidergic system in an experimental murine model of inflammatory orofacial pain.
      Borsani, Elisa, et al.
      J. Neurosci. Res., 88: 2715-26 (2010)  2010

      Show Abstract
      20648657 20648657
      Distinct pattern of microglial response, cyclooxygenase-2, and inducible nitric oxide synthase expression in the aged rat brain after excitotoxic damage.
      O Campuzano,M M Castillo-Ruiz,L Acarin,B Castellano,B Gonzalez
      Journal of neuroscience research  86  2008

      Show Abstract
      18543338 18543338
      Hepatocyte survival in acute hepatitis is due to c-Jun/AP-1-dependent expression of inducible nitric oxide synthase.
      Hasselblatt, P; Rath, M; Komnenovic, V; Zatloukal, K; Wagner, EF
      Proceedings of the National Academy of Sciences of the United States of America  104  17105-10  2007

      Show Abstract Full Text Article
      17940019 17940019
      Nasal vaccination with a proteosome-based adjuvant and glatiramer acetate clears beta-amyloid in a mouse model of Alzheimer disease.
      Frenkel, D; Maron, R; Burt, DS; Weiner, HL
      The Journal of clinical investigation  115  2423-33  2005

      Show Abstract
      16100572 16100572


      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      White Paper- Modern Methods in Oxidative Stress Research (EMD)