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AB16311 Anti-Nitric Oxide Synthase II Antibody

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AB16311
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB16311
      ReplacesAB1631
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase II Antibody
      Alternate Names
      • NOS II
      • iNOS
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% Sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Nitric Oxide Synthase II Antibody is validated for use in WB, IC for the detection of Nitric Oxide Synthase II.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot: 5 μg/mL using chemiluminescent detection. Higher concentrations of the antibody may be required if colorimetric detection is used. Antibody dilution will also vary with the concentration of the antigen. Immunocytochemistry: Use at 2-5 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 1131-1144 of mouse macrophage NOS, coupled to KLH.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityReactive with mouse NOS-II (iNOS).
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
      Gene Symbol
      • NOS2A
      • iNOS
      • HEP-NOS
      • INOS
      • NOS
      • NOS2
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
      COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
      SIZE: 1153 amino acids; 131117 Da
      SUBUNIT: Homodimer. Binds SLC9A3R1.
      TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
      SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at 2-8°C in undiluted aliquots for up to 6 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase II Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase II Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NITRIC OXIDE SYNTHASE II (NOS-II; iNOS) -2782072 2782072
      RABBIT ANTI-NITRIC OXIDE SYNTHASE II (NOS-II; iNOS) AFFINITY PURIFIED POLYCLONAL ANTIBODY 2949942
      RABBIT ANTI-NITRIC OXIDE SYNTHASE II (NOS-II; iNOS) AFFINITY PURIFIED POLYCLONAL ANTIBODY 3109201
      RABBIT ANTI-NITRIC OXIDE SYNTHASE II (NOS-II; iNOS) AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2330588 2330588

      References

      Reference overviewApplicationSpeciesPub Med ID
      LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.
      Tina Ruggiero, Michele Trabucchi, Francesca De Santa, Simona Zupo, Brian D Harfe, Michael T McManus, M Geoff Rosenfeld, Paola Briata, Roberto Gherzi
      The FASEB journal : official publication of the Federation of American Societies for Experimental Biology  23  2898-908  2009

      Show Abstract
      Immunoblotting (Western)Mouse19423639 19423639
      Transient axonal injury in the absence of demyelination: a correlate of clinical disease in acute experimental autoimmune encephalomyelitis.
      Aboul-Enein, Fahmy, et al.
      Acta Neuropathol., 111: 539-47 (2006)  2006

      Show Abstract
      16718350 16718350
      Adenosine A2A receptor stimulation potentiates nitric oxide release by activated microglia.
      Josep Saura, Ester Angulo, Aroa Ejarque, Vicent Casadó, Josep M Tusell, Rosario Moratalla, Jiang-Fan Chen, Michael A Schwarzschild, Carme Lluis, Rafael Franco, Joan Serratosa
      Journal of neurochemistry  95  919-29  2005

      Show Abstract
      16092928 16092928
      Selective and antigen-dependent effects of myelin degeneration on central nervous system inflammation.
      Aboul-Enein, Fahmy, et al.
      J. Neuropathol. Exp. Neurol., 63: 1284-96 (2004)  2004

      15624765 15624765

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Title
      Anti-Nitric Oxide Synthase II - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies