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AB5380 Anti-Nitric Oxide Synthase I Antibody

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AB5380
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, Mk, R, ShIHC, WBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB5380
      ReplacesAB1552
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase I Antibody
      Alternate Names
      • NOS I
      • bNOS
      • nNOS
      References
      Product Information
      FormatSerum
      Control
      • Brain tissue
      PresentationRabbit Serum. Liquid containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Nitric Oxide Synthase I Antibody is validated for use in IH, WB for the detection of Nitric Oxide Synthase I.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:1,000-1:3,000 (ECL).

      Immunohistochemistry: 1:1,000-1:2,000 (ABC).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant human neuronal nitric oxide synthase.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Neuronal nitric oxide synthase (nNOS, NOS-I, bNOS). No cross reaction with iNOS and eNOS by immunohistochemistry.
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      • Rat
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS1
      • bNOS
      • nNOS
      • NC-NOS
      • N-NOS
      • IHPS1
      • NOS
      • EC 1.14.13.39
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29475 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1434 amino acids; 160970 Da
      SUBUNIT: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity).
      SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
      TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
      DOMAIN: SwissProt: P29475 The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles (By similarity).
      DISEASE: SwissProt: P29475 # Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction.
      SIMILARITY: Belongs to the NOS family. & Contains 1 flavodoxin-like domain. & Contains 1 PDZ (DHR) domain.
      Molecular Weight161 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase I Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase I Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) 2463616
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 2227119 2227119
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3169861 3169861
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3273628 3273628
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3385775 3385775
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3425561 3425561
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3469460 3469460
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3607161 3607161
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) - 3703618 3703618
      RABBIT ANTI-NEURONAL NITRIC OXIDE SYNTHASE (nNOS) -2701569 2701569

      References

      Reference overviewApplicationSpeciesPub Med ID
      Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
      Gańko, M; Całka, J
      Journal of molecular neuroscience : MN  54  188-98  2014

      Show Abstract
      ImmunohistochemistryPorcine24643520 24643520
      Characterization of neuronal populations in the human trigeminal ganglion and their association with latent herpes simplex virus-1 infection.
      Flowerdew, SE; Wick, D; Himmelein, S; Horn, AK; Sinicina, I; Strupp, M; Brandt, T; Theil, D; Hüfner, K
      PloS one  8  e83603  2013

      Show Abstract
      24367603 24367603
      Morphological, immunocytochemical, and functional characterization of esophageal enteric neurons in primary culture.
      Dong, H; Jiang, Y; Srinivasan, S; Mittal, RK
      American journal of physiology. Gastrointestinal and liver physiology  305  G129-38  2013

      Show Abstract
      23660501 23660501
      Mice overexpressing wild-type human alpha-synuclein display alterations in colonic myenteric ganglia and defecation.
      Wang, L; Magen, I; Yuan, PQ; Subramaniam, SR; Richter, F; Chesselet, MF; Taché, Y
      Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society  24  e425-36  2012

      Show Abstract
      22779732 22779732
      Reticular groove of the domestic ruminants: histochemical and immunocytochemical study.
      G Scala,L Maruccio
      Anatomia, histologia, embryologia  41  2012

      Show Abstract
      22506730 22506730
      Role of neural NO synthase (nNOS) uncoupling in the dysfunctional nitrergic vasorelaxation of penile arteries from insulin-resistant obese Zucker rats.
      Sánchez, A; Contreras, C; Martínez, MP; Climent, B; Benedito, S; García-Sacristán, A; Hernández, M; Prieto, D
      PloS one  7  e36027  2012

      Show Abstract
      22540017 22540017
      Effects of nitric oxide synthase-3 overexpression on post-translational modifications and cell survival in HepG2 cells.
      P Aguilar-Melero,G Ferrín,J Muntané
      Journal of proteomics  75  2012

      Show Abstract
      21968428 21968428
      Neurochemical phenotypes of myenteric neurons in the rhesus monkey.
      Ali Reza Noorian,Georgia M Taylor,Dana M Annerino,James G Greene
      The Journal of comparative neurology  519  2011

      Show Abstract
      21618236 21618236
      Tetrodotoxin- and resiniferatoxin-induced changes in paracervical ganglion ChAT- and nNOS-IR neurons supplying the urinary bladder in female pigs.
      Piotr Józef Burli?ski,S?awomir Gonkowski,Jaros?aw Ca?ka
      Acta veterinaria Hungarica  59  2011

      Show Abstract
      22079707 22079707
      A blueprint for the spatiotemporal origins of mouse hippocampal interneuron diversity.
      Tricoire, L; Pelkey, KA; Erkkila, BE; Jeffries, BW; Yuan, X; McBain, CJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  31  10948-70  2011

      Show Abstract
      21795545 21795545

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies