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AB1632 Anti-Nitric Oxide Synthase I Antibody

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AB1632
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, M, RELISA, WB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1632
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nitric Oxide Synthase I Antibody
      Alternate Names
      • NOS I
      • bNOS
      • nNOS
      References
      Product Information
      FormatAffinity Purified
      PresentationPeptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
      Key Applications
      • ELISA
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

      Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

      ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.
      HostRabbit
      SpecificityReactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.
      Species Reactivity
      • Bovine
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOS1
      • nNOS
      • NC-NOS
      • N-NOS
      • IHPS1
      • bNOS
      • NOS
      • EC 1.14.13.39
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29475 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter.
      COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.
      SIZE: 1434 amino acids; 160970 Da
      SUBUNIT: Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity).
      SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity).
      TISSUE SPECIFICITY: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
      DOMAIN: SwissProt: P29475 The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles (By similarity).
      DISEASE: SwissProt: P29475 # Genetic variations in NOS1 gene are associated with susceptibility to infantile hypertrophic pyloric stenosis type 1 (IHPS1) [MIM:179010]. IHPS has an incidence of 1-5 per 1'000 live births in whites and a marked preponderance of males to females (4:1). IHPS is the most frequent disorder requiring surgery in the first year of life. The disorder is characterized by hypertrophy and hyperplasia of the circular muscle layer of the pylorus, leading to persistent vomiting 2-12 weeks after birth. Defective pyloric relaxation and increased pyloric smooth muscle mass have been suggested to be responsible for gastric-outlet obstruction.
      SIMILARITY: Belongs to the NOS family. & Contains 1 flavodoxin-like domain. & Contains 1 PDZ (DHR) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase I Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase I Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS) - 3574385 3574385
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS) POLYCLONAL ANTIBODY 3101537
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS) POLYCLONAL ANTIBODY -
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS) POLYCLONAL ANTIBODY - 2211909 2211909
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS) POLYCLONAL ANTIBODY - 2270327 2270327
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I -2570705 2570705

      References

      Reference overviewPub Med ID
      Nitric oxide (NO) expression during annual reproductive activity in buffalo epididymis: a histochemical and immunocytochemical study.
      Gaetano Scala,Lucianna Maruccio
      Theriogenology  78  2012

      Show Abstract
      22406309 22406309
      Microvasculature of the buffalo (Bubalus bubalis) choroid plexuses: structural, histochemical, and immunocytochemical study.
      Scala G, Corona M, Langella E, Maruccio L
      Microsc Res Tech  74  67-75.  2011

      Show Abstract
      21181712 21181712
      Structural, histochemical and immunocytochemical study of the forestomach mucosa in domestic ruminants.
      Scala G, Corona M, Maruccio L
      Anatomia, histologia, embryologia  40  47-54. doi  2011

      Show Abstract
      21029150 21029150
      Molecular typing of epithelial ovarian carcinomas using inflammatory markers.
      Rouba Ali-Fehmi,Assaad Semaan,Sima Sethi,Haitham Arabi,Sudeshna Bandyopadhyay,Yaser R Hussein,Michael P Diamond,Ghasan Saed,Robert T Morris,Adnan R Munkarah
      Cancer  117  2011

      Show Abstract
      20818651 20818651
      Lateralization of hippocampal nitric oxide mediator system in people with Alzheimer disease, multi-infarct dementia and schizophrenia.
      Zdena Kristofiková,Iryna Kozmiková,Petra Hovorková,Jan Rícný,Petr Zach,Emmerich Majer,Jan Klaschka,Daniela Rípová
      Neurochemistry international  53  2008

      Show Abstract
      18647632 18647632
      Distribution of neuronal nitric oxide synthase-immunoreactive neurons in the cerebral cortex and hippocampus during postnatal development.
      Yoon Hee Chung, Yang Soo Kim, Won Bok Lee
      Journal of molecular histology  35  765-70  2004

      Show Abstract
      15609089 15609089
      Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase.
      Bredt, D S, et al.
      Nature, 351: 714-8 (1991)  1991

      Show Abstract
      1712077 1712077

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress (EMD)

      Technical Info

      Title
      White Paper- Modern Methods in Oxidative Stress Research (EMD)

      Data Sheet

      Title
      RABBIT ANTI-NITRIC OXIDE SYNTHASE I (NOS-I; bNOS; nNOS)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies