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482728 Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody

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482728-100UL
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      Description
      OverviewRecognizes the ~130 kDa iNOS protein.
      Catalogue Number482728
      Brand Family Calbiochem®
      SynonymsAnti-iNOS
      Application Data
      Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
      References
      ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
      Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
      Bredt, D.S., et al. 1991. Nature 351, 714.
      Product Information
      FormLiquid
      FormulationUndiluted serum.
      Positive controlRaw 264.7 cells treated with IFNγ and LPS
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesFrozen Sections (1:500)
      Immunoblotting (1:2000)
      Immunocytochemistry (1:500)
      Paraffin Sections (1:500)
      Application CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

      Recommended Procedure for Immunocytochemistry of Anti-iNOS:
      1. Grow cells in appropriate medium.
      2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
      3. Wash cells 3-5 min with phosphate buffered saline (PBS).
      4. Permeabilize with 2% saponin for 10 min at room temperature.
      5. Fix in 4% formalin for 10 min at room temperature.
      6. Wash 3-5 min with PBS.
      7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
      8. Wash 3-5 min with PBS.
      9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
      10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
      Biological Information
      Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb Certificates of Analysis

      TitleLot Number
      482728

      References

      Reference overview
      Lowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
      Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
      Bredt, D.S., et al. 1991. Nature 351, 714.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      SynonymsAnti-iNOS
      ApplicationFrozen Sections (1:500)
      Immunoblotting (1:2000)
      Immunocytochemistry (1:500)
      Paraffin Sections (1:500)
      Application Data
      Detection of mouse inducible nitric oxide synthase by immunoblotting. Sample: Whole cell lysate from RAW 264.7 cells induced with IFNγ and LPS. Primary antibody: Anti-Nitric Oxide Synthase (1131-1144), Inducible Rabbit pAb (Cat. No. 482728) (1:1000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~130 kDa inducible nitric oxide synthase (iNOS) protein.
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide (CKKGSALEERKATRL) corresponding to amino acids 1131-1144 of mouse macrophage inducible nitric oxide synthase (iNOS), conjugated to KLH
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlRaw 264.7 cells treated with IFNγ and LPS
      FormLiquid
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsExhibits no cross-reactivity with bNOS or eNOS. Variables associated with assay conditions will dictate the proper working dilution.

      Recommended Procedure for Immunocytochemistry of Anti-iNOS:
      1. Grow cells in appropriate medium.
      2. Induce NOS by pretreating cells for 16 h with fresh media containing either: no addition (control), interferon-γ (50 ng/ml), LPS (30 µg/ml) or interferon plus LPS.
      3. Wash cells 3-5 min with phosphate buffered saline (PBS).
      4. Permeabilize with 2% saponin for 10 min at room temperature.
      5. Fix in 4% formalin for 10 min at room temperature.
      6. Wash 3-5 min with PBS.
      7. Incubate for 20 min at room temperature with anti-iNOS diluted 1:500 in PBS.
      8. Wash 3-5 min with PBS.
      9. Incubate with biotinylated secondary antibody (i.e. biotin-conjugated goat anti-rabbit IgG, Cat. No. 401313). Wash; incubate with avidin-conjugated alkaline phosphatase (Cat. No. 189726 or 189732).
      10. Develop with Naphthol AS Phosphate/Fast Red Violet LB (Cat. No. 477758) or Naphthol AS Phosphate/New Fuchsin (Cat. No. 477759).
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesLowenstein, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 6711.
      Lyons, C., et al. 1992. J. Biol. Chem. 267, 6370.
      Bredt, D.S., et al. 1991. Nature 351, 714.