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MAB5556 Anti-Nicastrin Antibody

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MAB5556
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IP, WB, ICC M Ascites Monoclonal Antibody
      Description
      Catalogue NumberMAB5556
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nicastrin Antibody
      References
      Product Information
      FormatAscites
      PresentationAscites fluid. Liquid. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nicastrin Antibody is an antibody against Nicastrin for use in IP, WB & IC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern blot. The antibody recognizes a doublet of ~100-110 kDa. Suggested antibody dilution buffer is TBS with 0.1% Tween-20 and 5% non-fat milk powder. Suggested incubation time is overnight at 2-8°C or 1-2 hours at room temperature.

      Immunocytochemistry

      Immunoprecipitation. Suggested tissue/cell lysis buffer is 1% Triton X100 or 2% CHAPS. Suggested final reaction volume is 1000 μL with a final protein concentration in the reaction mix of 1 mg/mL. Suggested incubation time is overnight at 2-8°C with rotating. The antibody is known to co-precipitate in CHAPS: presenilin, Aph-1 and Pen-2. Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the C terminal 18 a.a. of mouse Nicastrin. Due to sequence similarity, it is expected that the antibody will react with most mammals.
      Clone9C3
      HostMouse
      SpecificityNicastrin.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer's disease; however, the nature of the encoded protein's role in Alzheimer's disease is not known for certain. Alternatively spliced transcript variants have been described, but their full-length nature has not been determined.
      Gene Symbol
      • NCSTN
      • APH2
      • nicastrin
      • RP11-517F10.1
      • ATAG1874
      • KIAA0253
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q92542 # Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex.
      SIZE: 709 amino acids; 78411 Da
      SUBUNIT: Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Binds to proteolytic processed C-terminal fragments C83 and C99 of the amyloid precursor protein (APP).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Widely expressed.
      SIMILARITY: SwissProt: Q92542 ## Belongs to the nicastrin family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nicastrin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nicastrin Antibody Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-NICASTRIN - 25005692500569
      MOUSE ANTI-NICASTRIN -27962542796254
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY3000913
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY3143272
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 21155562115556
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 21217482121748
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 24501852450185
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 21113862111386
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 21596532159653
      MOUSE ANTI-NICASTRIN MONOCLONAL ANTIBODY - 22739222273922

      References

      Reference overviewPub Med ID
      Identification of two novel synaptic γ-secretase associated proteins that affect amyloid β-peptide levels without altering Notch processing.
      Susanne Frykman,Yasuhiro Teranishi,Ji-Yeun Hur,Anna Sandebring,Natsuko Goto Yamamoto,Maria Ancarcrona,Takeshi Nishimura,Bengt Winblad,Nenad Bogdanovic,Sophia Schedin-Weiss,Takahiro Kihara,Lars O Tjernberg
      Neurochemistry international  61  2012

      Show Abstract
      22521230 22521230
      Identification of novel γ-secretase-associated proteins in detergent-resistant membranes from brain.
      Hur, JY; Teranishi, Y; Kihara, T; Yamamoto, NG; Inoue, M; Hosia, W; Hashimoto, M; Winblad, B; Frykman, S; Tjernberg, LO
      The Journal of biological chemistry  287  11991-2005  2012

      Show Abstract
      22315232 22315232
      Synaptic and endosomal localization of active gamma-secretase in rat brain.
      Frykman, S; Hur, JY; Frånberg, J; Aoki, M; Winblad, B; Nahalkova, J; Behbahani, H; Tjernberg, LO
      PloS one  5  e8948  2010

      Show Abstract
      20126630 20126630
      gamma-Secretase dependent production of intracellular domains is reduced in adult compared to embryonic rat brain membranes.
      Frånberg, J; Karlström, H; Winblad, B; Tjernberg, LO; Frykman, S
      PloS one  5  e9772  2010

      Show Abstract
      20333303 20333303
      Protein-sphingolipid interactions within cellular membranes.
      Haberkant, P; Schmitt, O; Contreras, FX; Thiele, C; Hanada, K; Sprong, H; Reinhard, C; Wieland, FT; Brügger, B
      Journal of lipid research  49  251-62  2008

      Show Abstract
      17906222 17906222
      Presenilin 1 mediates the turnover of telencephalin in hippocampal neurons via an autophagic degradative pathway.
      Esselens, Cary, et al.
      J. Cell Biol., 166: 1041-54 (2004)  2004

      Show Abstract
      15452145 15452145