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MAB5446 Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24

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MAB5446
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RELISA, IP, WB, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB5446
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24
      References
      Product Information
      FormatAscites
      Control
      • Positive Control: Brain
      PresentationAscites fluid. Liquid.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 detects level of Neurofilament NF-H & has been published & validated for use in ELISA, IP, WB, IH.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern blot. Reacts with the 200 kD NF-H from human.

      Immunohistochemistry. The antibody has been used successfully on Bouin's and alcohol-fixed paraffin-embedded or frozen tissue sections. Formalin-fixed paraffin-embedded tissue sections require pretreatment with trypsin.

      Immunoprecipitation

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRat Neurofilaments
      Epitopetail domain (Phosphate dependent)
      CloneRMO-24
      HostMouse
      SpecificityReacts with Neurofilament Heavy (NF-H), tail domain (phosphate dependent). Dephosphorylation of the NF-H will results in diminished immunoreactivity.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFH
      • NFH
      • NF-H
      • KIAA0845
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P12036 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
      SIZE: 1026 amino acids; 112480 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      DISEASE: SwissProt: P12036 # Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
      SIMILARITY: SwissProt: P12036 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament NF-H Antibody, tail domain (Phosphate dependent), clone RMO-24 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) 2482263
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY - 2118953 2118953
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN -2679420 2679420
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY 3057119
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY - 2023673 2023673
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY - 2031334 2031334
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY - 2272689 2272689
      MOUSE ANTI-NEUROFILAMENT, HEAVY (NF-H) TAIL DOMAIN MONOCLONAL ANTIBODY -2504867 2504867

      References

      Reference overviewPub Med ID
      Monoclonal antibodies distinguish several differentially phosphorylated states of the two largest rat neurofilament subunits (NF-H and NF-M) and demonstrate their existence in the normal nervous system of adult rats.
      Lee, V M, et al.
      J. Neurosci., 7: 3474-88 (1987)  1987

      Show Abstract
      3119789 3119789

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      Life Science Research > Antibodies and Assays > Primary Antibodies