559844
Sigma-AldrichAnti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32)
This Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Neurofilament H Non-Phosphorylated.
More>>This Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) is validated for use in ELISA, Frozen Sections, WB, ICC, Paraffin Sections for the detection of Neurofilament H Non-Phosphorylated. Less<<
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Description
Overview
Recognizes the non-phosphorylated ~180 kDa-200 kDa neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations.
Catalogue Number
559844
Brand Family
Calbiochem®
References
References
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278. King, C.E., et al. 1997. Neuroreport.8, 1663. Campbell, M.J., et al. 1991. Brain Res.539, 133. Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191. Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
ELISA (1:1000) Frozen Sections (1:1000, see comments) Immunoblotting (1:1000, see comments) Immunocytochemistry (1:1000, see comments) Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Biological Information
Immunogen
homogenized hypothalami from Fischer 344 rat brain
Immunogen
Rat
Clone
SMI-32
Host
Mouse
Isotype
IgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Only recognizes non-phosphorylated neurofilament H. By immunocytochemistry this antibody stains neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems, but does not stain thin axons or other cells and tissues. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehye-containing fixatives, such as Bouin's fixative. Antibody reactivity is poor in glutaraldehyde/paraformaldehyde-fixed samples. For staining formalin-fixed, paraffin sections it is recommended that de-paraffinized tissue be autoclaved in dH₂O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min. Trypsin pre-treatment abolishes antibody binding to the epitope. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections or thick sections fixed in 4% paraformaldehyde and in cultured cells. By immunoblotting this antibody detects two bands, ~180 and ~200 kDa, which merge into a single NFH line on two-dimensional gels. Antibody should be titrated for optimal results in individual systems.
Packaging Information
Transport Information
Supplemental Information
Specifications
Documentation
Anti-Neurofilament H Non-Phosphorylated Mouse mAb (SMI-32) SDS
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278. King, C.E., et al. 1997. Neuroreport.8, 1663. Campbell, M.J., et al. 1991. Brain Res.539, 133. Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191. Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-May-2018 JSW
Application
ELISA (1:1000) Frozen Sections (1:1000, see comments) Immunoblotting (1:1000, see comments) Immunocytochemistry (1:1000, see comments) Paraffin Sections (1:1000, heat pre-treatment required, see comments)
Description
Mouse monoclonal antibody supplied as affinity purified antibody. Recognizes the ~180-200 kDa non-phosphorylated neurofilament H protein.
Background
Neurofilaments are a type of intermediate filament that serve as major constituent of the cytoskeleton of axons. They are the most abundant fibrillar components of the axon and are composed of three intertwined protofibrils. The neurofilament triplet proteins (NF-L, 68/70 kDa; NF-M, 160 kDa; and NF-H, 200 kDa) are found in both the central and peripheral nervous system and are usually neuron-specific. NF-H and NF-M both require the presence of NF-L protein to co-assemble. NF-H and NF-M become highly phosphorylated after newly formed neurofilaments enter the axon. Neurofilament staining is observed in neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas, and have also been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung are also known to express neurofilaments.
Host
Mouse
Immunogen species
Rat
Immunogen
homogenized hypothalami from Fischer 344 rat brain
Clone
SMI-32
Isotype
IgG₁
Species
mammalian
Form
Liquid
Formulation
In PBS
Storage
+2°C to +8°C
Avoid freeze/thaw
Do Not Freeze
Ok to freeze
Special Instructions
Only recognizes non-phosphorylated neurofilament H. By immunocytochemistry this antibody stains neuronal cell bodies, dendrites, and some thick axons in the central and peripheral nervous systems, but does not stain thin axons or other cells and tissues. Tissues and cultured cells can be fixed in a variety of paraformaldehyde- or formaldehye-containing fixatives, such as Bouin's fixative. Antibody reactivity is poor in glutaraldehyde/paraformaldehyde-fixed samples. For staining formalin-fixed, paraffin sections it is recommended that de-paraffinized tissue be autoclaved in dH₂O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min. Trypsin pre-treatment abolishes antibody binding to the epitope. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the neurons in frozen sections or thick sections fixed in 4% paraformaldehyde and in cultured cells. By immunoblotting this antibody detects two bands, ~180 and ~200 kDa, which merge into a single NFH line on two-dimensional gels. Antibody should be titrated for optimal results in individual systems.
Toxicity
Standard Handling
References
Trapp, B.D., et al. 1998. N. Engl. J. Med.338, 278. King, C.E., et al. 1997. Neuroreport.8, 1663. Campbell, M.J., et al. 1991. Brain Res.539, 133. Campbell, M.J., et al. 1989. J. Comp. Neurol.282, 191. Sternberger, L.A., et al. 1983. Proc. Natl. Acad. Sci. USA80, 6126.
