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MAB5254 Anti-Neurofilament 160 kDa Antibody, clone NN18

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MAB5254
40 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, Gp, M, Po, R, HIHC, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB5254
      ReplacesCBL214
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament 160 kDa Antibody, clone NN18
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatPurified
      Control
      • Brain
      • Cultured neurons
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament 160 kDa Antibody, clone NN18 is an antibody against Neurofilament 160 kDa for use in IH, IH(P) & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry:
      5-10 µg/mL on frozen or paraffin embedded tissue. See protocols for details.

      Western Blot:
      1:1000. 7.5%-10% SDS-PAGE

      Optimal working dilutions and protocols must be determined by end user.
      Biological Information
      ImmunogenPurified neurofilament polypeptides.
      CloneNN18
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with neurofilament 160 kD (Debus, E., 1982; Shaw, G., 1984).
      IsotypeIgG1
      Species Reactivity
      • Chicken
      • Guinea Pig
      • Mouse
      • Pig
      • Rat
      • Human
      Species Reactivity NoteExpected to react with human based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 916 amino acids; 102448 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
      Molecular Weight160 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
      Do not freeze.
      Packaging Information
      Material Size40 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament 160 kDa Antibody, clone NN18 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament 160 kDa Antibody, clone NN18 Certificates of Analysis

      TitleLot Number
      Anti-Neurofilament 160 kDa, clone NN18 - 2135111 2135111
      Anti-Neurofilament 160 kDa, clone NN18 - 2013407 2013407
      Anti-Neurofilament 160 kDa, clone NN18 - 2051863 2051863
      Anti-Neurofilament 160 kDa, clone NN18 - 2198956 2198956
      Anti-Neurofilament 160 kDa, clone NN18 - 2295656 2295656
      Anti-Neurofilament 160 kDa, clone NN18 - 2363000 2363000
      Anti-Neurofilament 160 kDa, clone NN18 - 3172467 3172467
      Anti-Neurofilament 160 kDa, clone NN18 - 3557955 3557955
      Anti-Neurofilament 160 kDa, clone NN18 - 3750050 3750050
      Anti-Neurofilament 160 kDa, clone NN18 - LV1481443 LV1481443

      References

      Reference overviewApplicationPub Med ID
      Regulatory mechanisms of testosterone-stimulated song in the sensorimotor nucleus HVC of female songbirds.
      Dittrich, F; Ramenda, C; Grillitsch, D; Frankl-Vilches, C; Ko, MC; Hertel, M; Goymann, W; ter Maat, A; Gahr, M
      BMC neuroscience  15  128  2014

      Show Abstract
      25442096 25442096
      Ablation of proliferating cells in the CNS exacerbates motor neuron disease caused by mutant superoxide dismutase.
      Audet, JN; Gowing, G; Paradis, R; Soucy, G; Julien, JP
      PloS one  7  e34932  2012

      Show Abstract
      22523565 22523565
      Decreased expression of neuronal nitric oxide synthase in the nucleus tractus solitarii inhibits sympathetically mediated baroreflex responses in rat.
      Lin, LH; Nitschke Dragon, D; Jin, J; Tian, X; Chu, Y; Sigmund, C; Talman, WT
      The Journal of physiology  590  3545-59  2012

      Show Abstract
      22687614 22687614
      The BTB and CNC homology 1 (BACH1) target genes are involved in the oxidative stress response and in the control of the cell cycle
      Warnatz HJ, Schmidt D, Manke T, Piccini I, Sultan M, Borodina T, Balzereit D, Wruck W, Soldatov A, Vingron M, Lehrach H, Yaspo ML
      J Biol Chem  2011

      Show Abstract
      21555518 21555518
      Sensory-motor deficits and neurofilament disorganization in gigaxonin-null mice.
      Ganay, T; Boizot, A; Burrer, R; Chauvin, JP; Bomont, P
      Molecular neurodegeneration  6  25  2011

      Show Abstract Full Text Article
      21486449 21486449
      Ablation of proliferating microglia does not affect motor neuron degeneration in amyotrophic lateral sclerosis caused by mutant superoxide dismutase.
      Gowing, G; Philips, T; Van Wijmeersch, B; Audet, JN; Dewil, M; Van Den Bosch, L; Billiau, AD; Robberecht, W; Julien, JP
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  10234-44  2008

      Show Abstract
      18842883 18842883
      Extended secondhand tobacco smoke exposure induces plasticity in nucleus tractus solitarius second-order lung afferent neurons in young guinea pigs.
      Shin-Ichi Sekizawa,Chao-Yin Chen,Andrea G Bechtold,Jocelyn M Tabor,John M Bric,Kent E Pinkerton,Jesse P Joad,Ann C Bonham
      The European journal of neuroscience  28  2008

      Show Abstract
      18657181 18657181
      Conditional NF-L transgene expression in mice for in vivo analysis of turnover and transport rate of neurofilaments.
      Millecamps, S; Gowing, G; Corti, O; Mallet, J; Julien, JP
      The Journal of neuroscience : the official journal of the Society for Neuroscience  27  4947-56  2007

      Show Abstract
      Western Blotting17475803 17475803
      Postganglionic nerve stimulation induces temporal inhibition of excitability in rabbit sinoatrial node.
      Fedorov, VV; Hucker, WJ; Dobrzynski, H; Rosenshtraukh, LV; Efimov, IR
      American journal of physiology. Heart and circulatory physiology  291  H612-23  2006

      Show Abstract
      16565321 16565321
      The immunological relatedness of neurofilament proteins of higher vertebrates.
      Shaw, G, et al.
      Eur. J. Cell Biol., 34: 130-6 (1984)  1984

      Show Abstract
      6203748 6203748

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      Life Science Research > Antibodies and Assays > Primary Antibodies