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MAB1621 Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11

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MAB1621
300 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Av, MaWB, ICC, IHCMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1621
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neurofilament 145 kDa Antibody, CT, clone 3H11
      Background InformationNeurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified antibody in PBS, 50% glycerol, and 5 mM sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Neurofilament 145 kDa Antibody, C-terminus, clone 3H11 detects level of Neurofilament 145 kDa & has been published & validated for use in WB, IC, IH.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:50-1:1000; fresh frozen, 4% PFA fixed tissue sections

      Immunoblotting: 1:1000 - 1:2000 ECL detection.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRecombinant fusion protein containing the last 168 amino acids of rat NF-M. Purified from E. Coli inclusion bodies by ion exchange chromatography on DEAE-cellulose in 6 M urea.
      EpitopeC-terminus
      Clone3H11
      HostMouse
      SpecificityReacts with an epitope dependent on the last 84 amino acids of rat NF-M (145K). Reacts specifically with NF-M from a variety of mammalian and avian species. The antibody is not sensitive to the phosphorylation state of the neurofilament.
      IsotypeIgG1
      Species Reactivity
      • Avian
      • Mammals
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NEFM
      • NF-M
      • NEF3
      • NFM
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07197 # Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
      SIZE: 916 amino acids; 102448 Da
      PTM: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. & Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
      SIMILARITY: SwissProt: P07197 ## Belongs to the intermediate filament family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size300 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neurofilament 145 kDa Antibody, CT, clone 3H11 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-NEUROFILAMENT M (145K) MONOCLONAL ANTIBODY - 2365948 2365948
      MOUSE ANTI-NEUROFILAMENT M (145K) MONOCLONAL ANTIBODY - 2428533 2428533
      MOUSE ANTI-NEUROFILAMENT M (145K) - 3201696 3201696
      MOUSE ANTI-NEUROFILAMENT M (145K) - 3380830 3380830
      MOUSE ANTI-NEUROFILAMENT M (145K) - 3762523 3762523
      MOUSE ANTI-NEUROFILAMENT M (145K) - 3862669 3862669
      MOUSE ANTI-NEUROFILAMENT M (145K) -2755743 2755743
      MOUSE ANTI-NEUROFILAMENT M (145K) MONOCLONAL ANTIBODY 3083124
      MOUSE ANTI-NEUROFILAMENT M (145K) MONOCLONAL ANTIBODY 2879765
      MOUSE ANTI-NEUROFILAMENT M (145K) MONOCLONAL ANTIBODY - 2055953 2055953

      References

      Reference overviewApplicationSpeciesPub Med ID
      Differential calcium signaling mediated by voltage-gated calcium channels in rat retinal ganglion cells and their unmyelinated axons.
      Sargoy, A; Sun, X; Barnes, S; Brecha, NC
      PloS one  9  e84507  2014

      Show Abstract
      ImmunohistochemistryRat24416240 24416240
      Therapeutic laquinimod treatment decreases inflammation, initiates axon remyelination, and improves motor deficit in a mouse model of multiple sclerosis.
      Moore, S; Khalaj, AJ; Yoon, J; Patel, R; Hannsun, G; Yoo, T; Sasidhar, M; Martinez-Torres, L; Hayardeny, L; Tiwari-Woodruff, SK
      Brain and behavior  3  664-82  2013

      Show Abstract
      24363970 24363970
      Channel density and porosity of degradable bridging scaffolds on axon growth after spinal injury.
      Thomas, AM; Kubilius, MB; Holland, SJ; Seidlits, SK; Boehler, RM; Anderson, AJ; Cummings, BJ; Shea, LD
      Biomaterials  34  2213-20  2013

      Show Abstract
      23290832 23290832
      Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury.
      Thomas, AM; Shea, LD
      Journal of controlled release : official journal of the Controlled Release Society  170  421-9  2013

      Show Abstract
      23791981 23791981
      Clusters of iron-rich cells in the upper beak of pigeons are macrophages not magnetosensitive neurons.
      Christoph Daniel Treiber,Marion Claudia Salzer,Johannes Riegler,Nathaniel Edelman,Cristina Sugar,Martin Breuss,Paul Pichler,Herve Cadiou,Martin Saunders,Mark Lythgoe,Jeremy Shaw,David Anthony Keays
      Nature  484  2012

      Show Abstract
      22495303 22495303
      Biochip∕laser cell deposition system to assess polarized axonal growth from single neurons and neuron∕glia pairs in microchannels with novel asymmetrical geometries.
      Pirlo, RK; Sweeney, AJ; Ringeisen, BR; Kindy, M; Gao, BZ
      Biomicrofluidics  5  13408  2011

      Show Abstract
      21522498 21522498
      Human embryonic stem cell neural differentiation and enhanced cell survival promoted by hypoxic preconditioning.
      Francis, KR; Wei, L
      Cell death & disease  1  e22  2010

      Show Abstract Full Text Article
      21364630 21364630
      Involvement of TRPV2 activation in intestinal movement through nitric oxide production in mice.
      Mihara, H; Boudaka, A; Shibasaki, K; Yamanaka, A; Sugiyama, T; Tominaga, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  16536-44  2010

      Show Abstract
      21147993 21147993
      Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1.
      Jarjour, AA; Bull, SJ; Almasieh, M; Rajasekharan, S; Baker, KA; Mui, J; Antel, JP; Di Polo, A; Kennedy, TE
      The Journal of neuroscience : the official journal of the Society for Neuroscience  28  11003-14  2008

      Show Abstract
      18945908 18945908
      Reduced expression of A-type potassium channels in primary sensory neurons induces mechanical hypersensitivity.
      Chien, LY; Cheng, JK; Chu, D; Cheng, CF; Tsaur, ML
      The Journal of neuroscience : the official journal of the Society for Neuroscience  27  9855-65  2007

      Show Abstract
      17855600 17855600

      Data Sheet

      Title
      MOUSE ANTI-NEUROFILAMENT M (145K)

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      Life Science Research > Antibodies and Assays > Primary Antibodies