AB5458 Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep)

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AB5458
50 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R IHC, WB Rb Serum Polyclonal Antibody
      Description
      Catalogue NumberAB5458
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Neprilysin Antibody (Neutral Endopeptidase, Nep)
      Alternate Names
      • neprilysin
      • Neprilysin
      • Enkephalinase
      • enkephalinase
      • atriopeptidase
      • Atriopeptidase
      • Neutral Endopeptidase
      • NEP
      • Endopeptidase 24.11
      • CD10
      Background InformationHuman neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.
      References
      Product Information
      FormatSerum
      Control
      • Kidney tissue.
      PresentationRabbit polyclonal serum. Contains no preservative.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blotting:
      A previous lot of this antibody was used on Western Blotting.

      Immunohistochemistry:
      A previous lot of this antibody was used on Immunohistochemistry.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat Neprilysin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteReacts with Human, Mouse, Rat. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
      Gene Symbol
      • MME
      • EPN
      • NEP
      • MGC126681
      • CD10
      • MGC126707
      • DKFZp686O16152
      • CALLA
      • EC 3.4.24.11
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08473 # Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Involved in the degradation of atrial natriuretic factor (ANF).
      COFACTOR: Binds 1 zinc ion per subunit.
      SIZE: 750 amino acids; 85514 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
      DISEASE: SwissProt: P08473 # Important cell surface marker in the diagnostic of human acute lymphocytic leukemia.
      SIMILARITY: SwissProt: P08473 ## Belongs to the peptidase M13 family.
      Molecular Weight~86 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) Certificates of Analysis

      TitleLot Number
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 21507112150711
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 24265212426521
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 19722721972272
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 20666752066675
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 22837942283794
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - 32999543299954
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - JC1668398JC1668398
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - LV1505474LV1505474
      Anti-Neprilysin (Neutral Endopeptidase, Nep) - NG1740931NG1740931
      Anti-Neprilysin (Neutral Endopeptidase, Nep) -28348132834813

      References

      Reference overviewApplicationPub Med ID
      Neurochemical profile of dementia pugilistica.
      Kokjohn, TA; Maarouf, CL; Daugs, ID; Hunter, JM; Whiteside, CM; Malek-Ahmadi, M; Rodriguez, E; Kalback, W; Jacobson, SA; Sabbagh, MN; Beach, TG; Roher, AE
      Journal of neurotrauma  30  981-97  2013

      Show Abstract
      Western Blotting23268705 23268705
      Isoflurane-induced spatial memory impairment by a mechanism independent of amyloid-beta levels and tau protein phosphorylation changes in aged rats.
      Liu, W; Xu, J; Wang, H; Xu, C; Ji, C; Wang, Y; Feng, C; Zhang, X; Xu, Z; Wu, A; Xie, Z; Yue, Y
      Neurological research  34  3-10  2012

      Show Abstract
      22196855 22196855
      The expression of renin-angiotensin system components in the human gastric mucosa.
      Hallersund, P; Elfvin, A; Helander, HF; Fändriks, L
      Journal of the renin-angiotensin-aldosterone system : JRAAS  12  54-64  2011

      Show Abstract
      20739374 20739374
      Alzheimer's disease and non-demented high pathology control nonagenarians: comparing and contrasting the biochemistry of cognitively successful aging.
      Maarouf, CL; Daugs, ID; Kokjohn, TA; Walker, DG; Hunter, JM; Kruchowsky, JC; Woltjer, R; Kaye, J; Castaño, EM; Sabbagh, MN; Beach, TG; Roher, AE
      PloS one  6  e27291  2011

      Show Abstract
      22087282 22087282
      Focal cerebral ischemia in rats alters APP processing and expression of Abeta peptide degrading enzymes in the thalamus.
      Mikko Hiltunen, Petra Mäkinen, Sirpa Peräniemi, Juhani Sivenius, Thomas van Groen, Hilkka Soininen, Jukka Jolkkonen, Mikko Hiltunen, Petra Mäkinen, Sirpa Peräniemi, Juhani Sivenius, Thomas van Groen, Hilkka Soininen, Jukka Jolkkonen, Mikko Hiltunen, Petra Mäkinen, Sirpa Peräniemi, Juhani Sivenius, Thomas van Groen, Hilkka Soininen, Jukka Jolkkonen
      Neurobiology of disease  35  103-13  2009

      Show Abstract
      19426802 19426802
      The epigenetic effects of amyloid-beta(1-40) on global DNA and neprilysin genes in murine cerebral endothelial cells.
      Kun-Lin Chen,Steven Sheng-Shih Wang,Yi-Yuan Yang,Rey-Yue Yuan,Ruei-Ming Chen,Chaur-Jong Hu
      Biochemical and biophysical research communications  378  2009

      Show Abstract
      19007750 19007750
      Increased expression of Abeta degrading enzyme IDE in the cortex of transgenic mice with Alzheimer's disease-like neuropathology.
      Saila Vepsäläinen, Mikko Hiltunen, Seppo Helisalmi, Jun Wang, Thomas van Groen, Heikki Tanila, Hilkka Soininen
      Neuroscience letters  438  216-20  2008

      Show Abstract
      18455870 18455870

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