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MABS2056-100UL Anti-Nardilysin (NRDC) Antibody, clone 102

MABS2056-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS2056-100UL
      DescriptionAnti-Nardilysin (NRDC) Antibody, clone 102
      Alternate Names
      • N-arginine dibasic convertase
      • NRD convertase
      • NRDC
      • NRD-C
      • Nardilysin convertase
      Background InformationNardilysin (UniProt: O43847; also known as EC: 3.4.24.61, N-arginine dibasic convertase, NRD convertase, NRD-C, Nardilysin convertase) is encoded by the NRDC (also known as NRD1) gene (Gene ID: 4898) in human. NRD-C is a metalloendopeptidase of the M16 family that cleaves peptides on the N-terminus of arginine residues in dibasic pairs. It is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off in the mature form. It promotes ecto-domain shedding of the precursor forms of various growth factors and cytokines by enhancing the protease activities of ADAM proteins. NRD-C is reported to bind to the extracellular domain of ADAM17 and directly enhance its catalytic activity. Its levels are shown to be elevated in serum and epithelium of gastric cancer and NRDC knockdown attenuates gastric cancer cell growth in vitro and in xenograft models. In mitochondria, it serves as a co-chaperone for proper folding of alpha-ketoglutarate dehydrogenase (alpha-KGDH) and absence of NRDC can lead to a severe reduction of alpha-KGDH. NRD-C is shown to inhibit pancreatitis and suppresses pancreatic ductal adenocarcinoma in mice. Its deletion in pancreatic tissue is reported to leads to spontaneous chronic pancreatitis concomitant with acinar-to-ductal conversion, increased apoptosis, and pancreatic atrophy in mice. (Ref.: Kanda, K., et al. (2012). EMBO Mol. Med. 4(5); 396-411; Yoon WH., et al. (2017). Neuron 93(1); 115-131; Ikuda, K., et al (2018). Gut (In Press; 10.1136/gutjnl-2017-315425).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Nardilysin (NRDC), clone 102, Cat. No. MABS2056, is a mouse monoclonal antibody that detects Nardilysin (NRDC) and has been tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Nardilysin (NRDC) in Western Blotting applications (Kanda, K., et. al. (2012). EMBO Mol Med. 4(5):396-411).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Nardilysin (NRDC) in Immunoprecipitation applications (Ohno, M., et. al. (2014). Neurobiol Aging. 35(1):213-22).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 80 amino acids from the N-terminal of human Nardilysin.
      Clone102
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 102 is a mouse monoclonal antibody that specifically detects human Nardilysin. It targets an epitope within 80 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NRDC
      • NRD1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed; 131.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human testis tissue lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nardilysin (NRDC) in human testis tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Nardilysin (NRDC) Antibody, clone 102 Certificates of Analysis

      TitleLot Number
      Anti-Nardilysin (NRDC), clone 102 - Q3237236Q3237236

      References

      Reference overviewPub Med ID
      Nardilysin prevents amyloid plaque formation by enhancing α-secretase activity in an Alzheimer's disease mouse model.
      Ohno, M; Hiraoka, Y; Lichtenthaler, SF; Nishi, K; Saijo, S; Matsuoka, T; Tomimoto, H; Araki, W; Takahashi, R; Kita, T; Kimura, T; Nishi, E
      Neurobiol Aging  35  213-22  2014

      Show Abstract
      23954170 23954170
      Nardilysin and ADAM proteases promote gastric cancer cell growth by activating intrinsic cytokine signalling via enhanced ectodomain shedding of TNF-α.
      Kanda, K; Komekado, H; Sawabu, T; Ishizu, S; Nakanishi, Y; Nakatsuji, M; Akitake-Kawano, R; Ohno, M; Hiraoka, Y; Kawada, M; Kawada, K; Sakai, Y; Matsumoto, K; Kunichika, M; Kimura, T; Seno, H; Nishi, E; Chiba, T
      EMBO Mol Med  4  396-411  2012

      Show Abstract
      22351606 22351606