Millipore Sigma Vibrant Logo

16-243 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488

View Products on Sigmaaldrich.com
16-243
25 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, M, Mk, Po, R, Rb, XnFC, ICC, WBMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number16-243
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488
      References
      Product Information
      FormatAlexaFluor®488
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 488 is validated for use in FC, IC, WB for the detection of Na+/K+ ATPase α-1.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
      Cloneclone C464.6
      HostMouse
      SpecificitySpecific for the α-1 subunit isoform.
      IsotypeIgG1κ
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Monkey
      • Pig
      • Rat
      • Rabbit
      • Xenopus
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
      Gene Symbol
      • ATP1A3
      • RDP
      • IIIsubunit).
      • MGC13276
      • DYT12
      • Na
      • +K
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
      SIZE: 1013 amino acids; 111749 Da
      SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
      SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
      Molecular Weight112kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at 4°C from date of shipment
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      16-243 04053252369421

      Documentation

      Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 488 Certificates of Analysis

      TitleLot Number
      Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG1) - 2201352 2201352
      Anti-Na+/K+ ATPase -1, clone C464.6, Alexa Fluor#174; 488 conjugate - JBC1511992 JBC1511992
      Anti-Na+/K+ ATPase alpha;-1, clone C464.6, Alexa Fluorreg; 488 conjugate - 30509 30509
      Anti-Na+/K+ ATPase alpha;-1, clone C464.6, Alexa Fluorreg; 488 conjugate - 33606 33606
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate (mouse monoclonal IgG1κ) 2989530
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate (mouse monoclonal IgG1κ) - 4219124 4219124
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate - 2386969 2386969
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate - 2519268 2519268
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate - 3166549 3166549
      Anti-Na+/K+ ATPase α-1, clone C464.6, Alexa Fluor® 488 conjugate - 3218764 3218764

      References

      Reference overviewApplicationPub Med ID
      Antibody development and use in chromogenic and fluorescent immunostaining.
      Eamon Dubaissi,Niki Panagiotaki,Nancy Papalopulu,Peter D Vize
      Methods in molecular biology (Clifton, N.J.)  917  2012

      Show Abstract
      22956101 22956101
      Na/K-ATPase, endogenous digitalis like compounds and cancer development -- a hypothesis.
      Weidemann, Heidrun
      Front. Biosci., 10: 2165-76 (2005)  2005

      Show Abstract
      15970485 15970485
      Neuronal function and alpha3 isoform of the Na/K-ATPase.
      Dobretsov, Maxim and Stimers, Joseph R
      Front. Biosci., 10: 2373-96 (2005)  2005

      Show Abstract
      15970502 15970502
      C-peptide, Na+,K(+)-ATPase, and diabetes.
      Vague, P, et al.
      Exp. Diabesity Res., 5: 37-50 (2004)  2004

      Show Abstract
      15198370 15198370
      FXYD proteins: new tissue- and isoform-specific regulators of Na,K-ATPase.
      Geering, Käthi, et al.
      Ann. N. Y. Acad. Sci., 986: 388-94 (2003)  2003

      Show Abstract
      12763855 12763855
      ADP-ribosylation Factor-dependent Phospholipase D Activation by the M3 Muscarinic Receptor
      Mitchell, R., et al
      J Biol Chem, 278:33818-33830 (2003)  2003

      Immunoblotting (Western)12799371 12799371
      Chloride, not sodium, stimulates expression of the {gamma} subunit of Na/K-ATPase and activates JNK in response to hypertonicity in mouse IMCD3 cells
      Capasso, J. M., et al
      Proc Natl Acad Sci U S A, 100:6428-6433 (2003)  2003

      Immunoblotting (Western)12746499 12746499
      Muc4/Sialomucin Complex, the Intramembrane ErbB2 Ligand, Translocates ErbB2 to the Apical Surface in Polarized Epithelial Cells
      Ramsauer, V. P., et al
      J Biol Chem, 278:30142-7 (2003)  2003

      Immunoblotting (Western)12748185 12748185
      Na+-K+--ATPase-mediated signal transduction: from protein interaction to cellular function.
      Xie, Zijian and Cai, Ting
      Mol. Interv., 3: 157-68 (2003)  2003

      Show Abstract
      14993422 14993422
      Identification of a novel ankyrin isoform (AnkG190) in kidney and lung that associates with the plasma membrane and binds alpha-Na, K-ATPase
      Thevananther, S., et al
      J Biol Chem, 273:23952-8 (1998)  1998

      Immunoprecipitation9727010 9727010

      Related Products & Applications

      Related Products

      Catalog Number Description  
      05-369X-555 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555 Show Pricing & Availability

      Alternative Format

      Catalog Number Description  
      05-369 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 Show Pricing & Availability
      05-369X-555 Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor® 555 Show Pricing & Availability

      Included Negative Control

      Catalog Number Description  
      16-240 Normal Mouse IgG, Alexa Fluor® 488 conjugate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies