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AB1557P Anti-NMDAR2B Antibody

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AB1557P
10 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1557P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-NMDAR2B Antibody
      Alternate Names
      • N-methyl D-aspartate receptor subtype 2B
      • N-methyl-D-aspartate receptor subunit 2B
      • N-methyl-D-aspartate receptor subunit 3
      • glutamate receptor subunit epsilon-2
      • glutamate receptor. ionotropic
      • N-methyl D-aspartate 2B
      • Glutamate
      Background InformationNMDA receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D). GRIN2B may be a candidate gene for the neurodegenerative disorder dentato-rubro-pallidoluysian atrophy (DRPLA).
      Properties of NMDAR include modulation by glycine, inhibition by Zn2+, voltage dependent Mg2+ blockade and high Ca2+ permeability. The involvement of NMDAR in the CNS has become a focus area for neurodegenerative diseases such as Alzheimer's disease and also epilepsy and ischemic neuronal cell death.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue, Rat Purkinje cell dendrites, rat hippocampus lysate, HEK 293 cells expressing NR2B.
      PresentationPurified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM). Some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NMDAR2B Antibody is an antibody against NMDAR2B for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      1:1,000-1:2,000 dilution of a previous lot was used on immunohistochemistry.

      Immunoprecipitation:
      3 μL of a previous lot of this antibody (under appropriate conditions) quantitatively immunoprecipitated all NMDAR2B in 200 μg of rat brain.

      Western Blot Analysis:
      1:100-1:2,000 dilution of a previous lot (NMDAR2B is present in high concentrations in the hippocampus).

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenC-Terminal Fusion Protein of the NMDAR2B (30 kDa).
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityNMDAR2B. Strong and specific immunolabeling of the 180 kDa NMDAR2B subunit of the NMDA receptor. No reactivity to NMDAR2A or NMDAR2C by Western blot. Immunolabeling has been blocked by preadsorption of the antibody with the C-terminal fusion protein that was used to generate the antibody.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryN-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
      Gene Symbol
      • GRIN2B
      • MGC142180
      • NMDAR2B
      • NR3
      • NR2B
      • MGC142178
      • hNR3
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13224 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
      SIZE: 1484 amino acids; 166367 Da
      SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B). Found in a complex with GRIN1 and GRIN3B. Found in a complex with GRIN1, GRIN3A and PPP2CB. Interacts with PDZ domains of INADL and DLG4 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
      SIMILARITY: SwissProt: Q13224 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NMDAR2B Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NMDAR2B Antibody Certificates of Analysis

      TitleLot Number
      Anti-NMDAR2B 3072340
      Anti-NMDAR2B - 2120023 2120023
      Anti-NMDAR2B - 2387462 2387462
      Anti-NMDAR2B - 2458943 2458943
      Anti-NMDAR2B - 1986129 1986129
      Anti-NMDAR2B - 2021178 2021178
      Anti-NMDAR2B - 2055960 2055960
      Anti-NMDAR2B - 2207147 2207147
      Anti-NMDAR2B - 2318755 2318755
      Anti-NMDAR2B - 3169524 3169524

      References

      Reference overviewApplicationSpeciesPub Med ID
      Repeated neonatal propofol administration induces sex-dependent long-term impairments on spatial and recognition memory in rats.
      Gonzales, EL; Yang, SM; Choi, CS; Mabunga, DF; Kim, HJ; Cheong, JH; Ryu, JH; Koo, BN; Shin, CY
      Biomolecules & therapeutics  23  251-60  2015

      Show Abstract
      25995824 25995824
      Neurological effects of inorganic arsenic exposure: altered cysteine/glutamate transport, NMDA expression and spatial memory impairment.
      Ramos-Chávez, LA; Rendón-López, CR; Zepeda, A; Silva-Adaya, D; Del Razo, LM; Gonsebatt, ME
      Frontiers in cellular neuroscience  9  21  2015

      Show Abstract
      25709567 25709567
      Cross-modal plasticity results in increased inhibition in primary auditory cortical areas.
      Mao, YT; Pallas, SL
      Neural plasticity  2013  530651  2013

      Show Abstract
      24288625 24288625
      Protease-activated receptor-1 modulates hippocampal memory formation and synaptic plasticity.
      Almonte, AG; Qadri, LH; Sultan, FA; Watson, JA; Mount, DJ; Rumbaugh, G; Sweatt, JD
      Journal of neurochemistry  124  109-22  2013

      Show Abstract
      23113835 23113835
      Behavioral and structural responses to chronic cocaine require a feedforward loop involving ΔFosB and calcium/calmodulin-dependent protein kinase II in the nucleus accumbens shell.
      Robison, AJ; Vialou, V; Mazei-Robison, M; Feng, J; Kourrich, S; Collins, M; Wee, S; Koob, G; Turecki, G; Neve, R; Thomas, M; Nestler, EJ
      The Journal of neuroscience : the official journal of the Society for Neuroscience  33  4295-307  2013

      Show Abstract
      23467346 23467346
      Chronic alcohol remodels prefrontal neurons and disrupts NMDAR-mediated fear extinction encoding.
      Andrew Holmes,Paul J Fitzgerald,Kathryn P Macpherson,Lauren Debrouse,Giovanni Colacicco,Shaun M Flynn,Sophie Masneuf,Kristen E Pleil,Chia Li,Catherine A Marcinkiewcz,Thomas L Kash,Ozge Gunduz-Cinar,Marguerite Camp
      Nature neuroscience  15  2012

      Show Abstract
      22941108 22941108
      Genetic, pharmacological and lesion analyses reveal a selective role for corticohippocampal gLUN2B in a novel repeated swim stress paradigm.
      Kiselycznyk CL, Svenningsson P, Delpire E, Holmes A
      Neuroscience  2011

      Show Abstract
      21704131 21704131
      Lead exposure during synaptogenesis alters NMDA receptor targeting via NMDA receptor inhibition.
      April P Neal,Paul F Worley,Tomás R Guilarte
      Neurotoxicology  32  2011

      Show Abstract
      21192972 21192972
      Gestational nicotine exposure regulates expression of AMPA and NMDA receptors and their signaling apparatus in developing and adult rat hippocampus.
      Wang, H; Dávila-García, MI; Yarl, W; Gondré-Lewis, MC
      Neuroscience  188  168-81  2011

      Show Abstract
      Western Blotting21596105 21596105
      Oligodendrocytes are damaged by neuromyelitis optica immunoglobulin G via astrocyte injury.
      Marignier, R; Nicolle, A; Watrin, C; Touret, M; Cavagna, S; Varrin-Doyer, M; Cavillon, G; Rogemond, V; Confavreux, C; Honnorat, J; Giraudon, P
      Brain : a journal of neurology  133  2578-91  2010

      Show Abstract
      20688809 20688809

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer (EMD)

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies