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MABF2115-100UL Anti-NKR-P1B (CD161b) Antibody, clone 2D12

MABF2115-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2115-100UL
      DescriptionAnti-NKR-P1B (CD161b) Antibody, clone 2D12
      Alternate Names
      • Killer cell lectin-like receptor subfamily B member 1B allele B
      • CD161 antigen-like family member B
      • Inhibitory receptor NKR-P1B
      • Lymphocyte antigen 55d
      • Ly-55d
      • Natural killer cell surface protein NKR-P1B allele B6
      • CD161b
      Background InformationKiller cell lectin-like receptor subfamily B member 1B allele B (UniProt: Q99JB4; also known as CD161 antigen-like family member B, Inhibitory receptor NKR-P1B, Lymphocyte antigen 55d, Ly-55d, NKR-P1D, Natural killer cell surface protein NKR-P1B allele B6, CD161b) is encoded by the Klrb1b (also known as Klrb1d, Ly55d, Nkrp1b, Nkrp1d) gene (Gene ID: 80782) in murine species. NKR-P1B is a single-pass type II disulfide-linked homodimeric membrane protein that is expressed only on a subset of mature natural killer (NK) cells. It serves as an inhibitory receptor that inhibits NK cell function upon interaction with its cognate C-type lectin-related ligand, Clr-b. The NKR-P1B-Clr-b system plays a role in tumor surveillance and immune escape in a transgenic murine model of B-cell lymphoma. It is reported to mediate MHC class I-independent "missing-self" recognition of allografts, tumor cells, and virus-infected cells. Structurally, it has a cytoplasmic domain (aa 1-43), a short transmembrane domain (aa 44-64), and extracellular domain (aa 65-223). Its N-terminal ITIM motif (aa 6-11) undergoes phosphorylation and the phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases leading to down-regulation of cell activation. (Ref.: Kirkham, CL and Carlyle, JR (2014). Front. Immunol. 5; 214; Rahim, MMA et al., (2015). Blood. 125(14):2217-2227; Aguilar, OA et al., (2017). Cell 169(1); 58-71).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Applications
      ApplicationAnti-NKR-P1B (CD161b), clone 2D12, Cat. No. MABF2115, is a mouse monoclonal antibody that detects NKR-P1B (CD161b) and has been tested for use in Flow Cytometry, Function Analysis, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Function Assay
      • Immunoprecipitation
      • Western Blotting
      Application NotesAffects Function Analysis: A representative lot reversed the reduction in killing induced by the expression of Clrb in C1498, BW5147, and B6 LAK cells. (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated NKR-P1B (CD161b) in Immunoprecipitation applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7).

      Flow Cytometry Analysis: A representative lot detected NKR-P1B (CD161b) in Flow Cytometry applications (Iizuka, K., et. al. (2003). Nat Immunol. 4(8):801-7; Aguilar, O.A., et. al. (2017). Cell. 169(1):58-71).
      Biological Information
      ImmunogenMouse BaF/3 cells (murine IL-3-dependent pro-B cell line) expressing mouse NKR-P1B protein.
      Clone2D12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2D12 is a mouse monoclonal antibody that detects Natural killer cell surface protein NKR-P1B allele B6 (NKR-P1B) in murine species. It does not react with Nkrp1a, Nkrp1c, and Nkrp1f.
      IsotypeIgG2aλ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Klrb1b
      • Klrb1d
      • Ly55d
      • Nkrp1b
      • Nkrp1d
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~52 kDa observed; 25.03 kDa calculated. . Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse spleen tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NKR-P1B (CD161b) in mouse spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NKR-P1B (CD161b) Antibody, clone 2D12 Certificates of Analysis

      TitleLot Number
      Anti-NKR-P1B (CD161b), clone 2D12 - Q3238883Q3238883

      References

      Reference overviewPub Med ID
      A Viral Immunoevasin Controls Innate Immunity by Targeting the Prototypical Natural Killer Cell Receptor Family.
      Aguilar, OA; Berry, R; Rahim, MMA; Reichel, JJ; Popović, B; Tanaka, M; Fu, Z; Balaji, GR; Lau, TNH; Tu, MM; Kirkham, CL; Mahmoud, AB; Mesci, A; Krmpotić, A; Allan, DSJ; Makrigiannis, AP; Jonjić, S; Rossjohn, J; Carlyle, JR
      Cell  169  58-71.e14  2017

      Show Abstract
      28340350 28340350
      Genetically linked C-type lectin-related ligands for the NKRP1 family of natural killer cell receptors.
      Iizuka, K; Naidenko, OV; Plougastel, BF; Fremont, DH; Yokoyama, WM
      Nat Immunol  4  801-7  2003

      Show Abstract
      12858173 12858173