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MABS1949-25UG Anti-NG2 Antibody, clone 1E6

MABS1949-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1949-25UG
      DescriptionAnti-NG2 Antibody, clone 1E6
      Alternate Names
      • Chondroitin sulfate proteoglycan 4
      • Chondroitin sulfate proteoglycan NG2
      • Proteoglycan AN2
      Background InformationChondroitin sulfate proteoglycan 4 (UniProt: Q8VHY0; also known as Chondroitin sulfate proteoglycan NG2, Proteoglycan AN2) is encoded by the Cspg4 (also known as An2, Kiaa4232, Ng2) gene (Gene ID: 121021) in murine species. Chondroitin sulfate proteoglycan NG2 is a single-pass type I membrane protein that plays a role in cell proliferation and migration and stimulates endothelial cells motility during microvascular morphogenesis. It is present in neural cells and in extraneural tissues, especially in the developing mesenchyme. Its level of expression is highest on immature, proliferating cells and decreases when these cells begin to differentiate. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells. It is a major chondroitin sulfate proteoglycan that is produced after spinal cord injury and is expressed by macrophages and oligodendrocyte progenitors. It is also reported to inhibit neurite outgrowth and growth cone collapse during axon regeneration. NG2 is synthesized with a signal peptide (aa 1-29), which is subsequently cleaved off to produce the mature form that contains a large extracellular domain (aa 30-2229), a transmembrane domain (aa 2230-2250), and a cytoplasmic domain (aa 2251-2327). NG2 can be present in highly O-glycosylated state, which is required for proper localization and function in stress fiber formation. Its phosphorylation by protein kinase C alpha is reported to regulate its subcellular location and cell motility function. It is generally localized at the apical plasma membrane and it re-localizes to the lamellipodia of astrocytoma upon phosphorylation.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NG2, clone 1E6, Cat. No. MABS1949, is a rat monoclonal antibody that detects Chondroitin sulfate proteoglycan 4 (NG2) and has ben tested for use in ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated NG2 in immunoprecipitation applications (Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).

      Western Blotting Analysis: A representative lot detected NG2 in Western Blotting applications (Stegmuller, J., et. al. (2002). J Neurocytol. 31(6-7):497-505; Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Trotter, J., et. al. (2005). J Neurol Sci. 233(1-2):37-42; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).

      ELISA Analysis: A representative lot detected NG2 in ELISA applications (Maus, F., et. al. (2015). PLoS One. 10(9):e0137311).

      Immunofluorescence Analysis: A representative lot detected NG2 in Immunoprecipitation applications (Schneider, S., et. al. (2001). J Neurosci. 21(3):920-33; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61; Karram, K., et. al. (2005). J Anat. 207(6):735-44; Chatterjee, N., et. al. (2008). J Biol Chem. 283(13):8310-7).

      Immunohistochemistry Analysis: A representative lot detected NG2 in Immunohistochemistry applications (Karram, K., et. al. (2005). J Anat. 207(6):735-44; Niehaus, A., et. al. (1999). J Neurosci. 19(12):4948-61).
      Biological Information
      ImmunogenOli-Neu cells (murine oligodendroglial precursor cells immortalized by an activated neu tyrosine kinase).
      Clone1E6
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 1E6 is a rat monoclonal antibody that detects mouse Chondroitin sulfate proteoglycan 4 (NG2).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Cspg4
      • An2
      • Kiaa4232
      • Ng2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~330 kDa observed; 252.31 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysates.

      Western Blotting Analysis: 2 µg/mL of this antibody detected NG2 in mouse brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MABS1949-25UG 04061841099116

      Documentation

      Anti-NG2 Antibody, clone 1E6 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-NG2 Antibody, clone 1E6 Certificates of Analysis

      TitleLot Number
      Anti-NG2, clone 1E6 - 3308348 3308348

      References

      Reference overviewPub Med ID
      The NG2 Proteoglycan Protects Oligodendrocyte Precursor Cells against Oxidative Stress via Interaction with OMI/HtrA2.
      Maus, F; Sakry, D; Binamé, F; Karram, K; Rajalingam, K; Watts, C; Heywood, R; Krüger, R; Stegmüller, J; Werner, HB; Nave, KA; Krämer-Albers, EM; Trotter, J
      PLoS One  10  e0137311  2015

      Show Abstract
      26340347 26340347
      Interaction of syntenin-1 and the NG2 proteoglycan in migratory oligodendrocyte precursor cells.
      Chatterjee, N; Stegmüller, J; Schätzle, P; Karram, K; Koroll, M; Werner, HB; Nave, KA; Trotter, J
      J Biol Chem  283  8310-7  2008

      Show Abstract
      18218632 18218632
      NG2-positive cells in CNS function and the pathological role of antibodies against NG2 in demyelinating diseases.
      Trotter, J
      J Neurol Sci  233  37-42  2005

      Show Abstract
      15949494 15949494
      NG2-expressing cells in the nervous system: role of the proteoglycan in migration and glial-neuron interaction.
      Karram, K; Chatterjee, N; Trotter, J
      J Anat  207  735-44  2005

      Show Abstract
      16367801 16367801
      AN2, the mouse homologue of NG2, is a surface antigen on glial precursor cells implicated in control of cell migration.
      Stegmüller, J; Schneider, S; Hellwig, A; Garwood, J; Trotter, J
      J Neurocytol  31  497-505  2001

      Show Abstract
      14501219 14501219
      The AN2 protein is a novel marker for the Schwann cell lineage expressed by immature and nonmyelinating Schwann cells.
      Schneider, S; Bosse, F; D'Urso, D; Muller, H; Sereda, MW; Nave, K; Niehaus, A; Kempf, T; Schnolzer, M; Trotter, J
      J Neurosci  21  920-33  2001

      Show Abstract
      11157078 11157078
      Cell-surface glycoprotein of oligodendrocyte progenitors involved in migration.
      Niehaus, A; Stegmüller, J; Diers-Fenger, M; Trotter, J
      J Neurosci  19  4948-61  1999

      Show Abstract
      10366628 10366628