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MABC1713-100UG Anti-NAC1 Antibody, clone 9.27

MABC1713-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1713-100UG
      DescriptionAnti-NAC1 Antibody, clone 9.27
      Alternate Names
      • Nucleus accumbens-associated protein 1
      • BTB/POZ domain-containing protein 14B
      Background InformationNucleus accumbens-associated protein 1 (UniProt: Q96RE7; also known as NAC-1, BTB/POZ domain-containing protein 14B) is encoded by the NACC1 (also known as BTBD14B, NAC1) gene (Gene ID: 112939) in human. NAC-1 serves as a transcriptional repressor in neuronal cells through the recruitment of HDAC3 and HDAC4. It contains a BTB domain (aa 30-94; Broad Complex, Tramtrack and Bric à brac) at its N-terminal and a BEN domain (aa 374-471; BANP, E5R and NAC1) in its C-terminal region. NAC1 undergoes homodimerization through its BTB domain. It differs from other BTB-domain transcription factors in that it lacks a characteristic zinc finger or basic leucine-zipper DNA-binding domain. NAC-1 is shown to be important in maintaining pluripotency of embryonic stem cells. It can direct bind to Nanog and also regulates the expression of Nanog, Oct4, and Sox-2. NAC-1 is reported to be overexpressed in several types of human carcinomas, including ovarian serous, pancreatic, and breast carcinoma. It contributes to tumor progression, tumor cell proliferation, and survival through repressing transcriptional activity of GADD45GIP1. NAC-1 knock-down can result in the apoptosis of ovarian cancer cell lines and enhance their chemosensitivity. (Ref.: Okazaki, K., et al. (2012). Carcinogenesis. 33(10); 1854 1862; Nakayama, N., et al. (2018). Oncotarget. 9(47); 28408-28420).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NAC1, clone 9.27, Cat. No. MABC1713, is a mouse monoclonal antibody that detects Nucleus accumbens-associated protein 1 (NAC1) and has been tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected NAC1 in human ovarian cancer tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected NAC1 in Immunohistochemistry applications (Okazaki, K., et. al. (2012), Carcinogenesis. 33(10):1854-62).

      Western Blotting Analysis: A representative lot detected NAC1 in Western Blotting applications (Okazaki, K., et. al. (2012), Carcinogenesis. 33(10):1854-62).

      Enzyme Immunoassay Analysis: A representative lot detected NAC1 in ELISA applications (Okazaki, K., et. al. (2012), Carcinogenesis. 33(10):1854-62).

      Immunocytochemistry Analysis: A representative lot detected NAC1 in Immunocytochemistry applications (Okazaki, K., et. al. (2012), Carcinogenesis. 33(10):1854-62).
      Biological Information
      ImmunogenGST-tagged full-length recombinant human NAC-1 protein.
      Clone9.27
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9.27 is a mouse monoclonal antibody that detects Nucleus accumbens-associated protein 1 (NAC-1).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NACC1
      • NAC1
      • BTBD14B
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight57.26 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human colon tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected NAC1 in human colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-NAC1 Antibody, clone 9.27 Certificates of Analysis

      TitleLot Number
      Anti-NAC1, clone 9.27 - Q3246230Q3246230

      References

      Reference overviewPub Med ID
      Nuclear localization signal in a cancer-related transcriptional regulator protein NAC1.
      Okazaki, K; Nakayama, N; Nariai, Y; Nakayama, K; Miyazaki, K; Maruyama, R; Kato, H; Kosugi, S; Urano, T; Sakashita, G
      Carcinogenesis  34  1854-63  2013

      Show Abstract
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