ABE251 Anti-N-CoR Antibody

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ABE251
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M WB Rb Affinity Purified Polyclonal Antibody
      Description
      Catalogue NumberABE251
      Replaces06-892
      DescriptionAnti-N-CoR Antibody
      Alternate Names
      • Nuclear receptor corepressor 1
      • N-CoR
      • N-CoR1
      • Retinoid X receptor-interacting protein 13
      Background InformationIn the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • RAW 264.7 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-N-CoR Antibody is a Rabbit Polyclonal Antibody for detection of N-CoR also known as Nuclear receptor corepressor 1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ncor1
      • Rxrip13
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.

      SUBUNIT STRUCTURE: Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with HEXIM1 By similarity. Interacts with C1D, SIAH2, HDAC7, SAP30, SIN3A and SIN3B. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Interacts with DACH1. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2.

      SUBCELLULAR LOCATION: Nucleus.
      Molecular Weight~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in RAW 264.7 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected N-CoR in RAW 264.7 cell lysate. No tween for best results.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-N-CoR Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-N-CoR Antibody Certificates of Analysis

      TitleLot Number
      Anti-N-CoR - 23840112384011
      Anti-N-CoR - 19596671959667
      Anti-N-CoR - 20740692074069
      Anti-N-CoR - 21530592153059
      Anti-N-CoR - 22712162271216
      Anti-N-CoR - 32980003298000
      Anti-N-CoR - NRG1911089NRG1911089
      Anti-N-CoR -24954802495480
      Anti-N-CoR -27363702736370
      Anti-N-CoR -28077692807769

      References

      Reference overviewPub Med ID
      Trichostatin A modulates thiazolidinedione-mediated suppression of tumor necrosis factor α-induced lipolysis in 3T3-L1 adipocytes.
      Lu, JC; Chang, YT; Wang, CT; Lin, YC; Lin, CK; Wu, ZS
      PloS one  8  e71517  2013

      Show Abstract
      23951179 23951179

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies