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AB16527 Anti-MyD88 Antibody, CT

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AB16527
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB16527
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MyD88 Antibody, CT
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified immunoglobulin. Liquid in PBS containing 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MyD88 Antibody, C-terminus is an antibody against MyD88 for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern blot: 1:500 to 1:1000

      Whole cell lysate from Jurkat cells can be used as positive control and a 35 kDa band should be detected.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenRabbit anti-MyD88 (C-terminus) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 296 of human MyD88 (Bonnert et al. 1997), which are identical to those of mouse.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (Muzio et al. 1997). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R mediated NF-kB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (Adachi et al. 1998; Medzhitov et al. 1998), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in loss of cellular responses to IL-1 and IL-18, and MyD88-defecient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYD88
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99836 # Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. May be involved in myeloid differentiation.
      SIZE: 296 amino acids; 33233 Da
      SUBUNIT: Homodimer. Also forms heterodimers with TIRAP. Binds to TLR2, TLR4, IRAK1 and IRAK2 via their respective TIR domains. Interacts with IL1RL1.
      SUBCELLULAR LOCATION: Cytoplasm (Probable).
      TISSUE SPECIFICITY: Ubiquitous.
      SIMILARITY: SwissProt: Q99836 ## Contains 1 death domain. & Contains 1 TIR domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      AB16527 04053252268618

      Documentation

      Anti-MyD88 Antibody, CT SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MyD88 Antibody, CT Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-MyD88 (C-TERMINUS) - 3669256 3669256
      RABBIT ANTI-MyD88 (C-TERMINUS) - 3868689 3868689
      RABBIT ANTI-MyD88 (C-TERMINUS) -2767261 2767261
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY 2976328
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY 2901148
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY 2869795
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY 2884721
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY - 2051852 2051852
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY - 2328306 2328306
      RABBIT ANTI-MyD88 (C-TERMINUS) POLYCLONAL ANTIBODY -2488763 2488763

      References

      Reference overviewApplicationPub Med ID
      The gammaherpesviruses Kaposi's sarcoma-associated herpesvirus and murine gammaherpesvirus 68 modulate the Toll-like receptor-induced proinflammatory cytokine response.
      Bussey, KA; Reimer, E; Todt, H; Denker, B; Gallo, A; Konrad, A; Ottinger, M; Adler, H; Stürzl, M; Brune, W; Brinkmann, MM
      Journal of virology  88  9245-59  2014

      Show Abstract
      24899179 24899179
      Naturally occurring mutation affecting the MyD88-binding site of TNFRSF13B impairs triggering of class switch recombination.
      Almejun, MB; Cols, M; Zelazko, M; Oleastro, M; Cerutti, A; Oppezzo, P; Cunningham-Rundles, C; Danielian, S
      European journal of immunology  43  805-14  2013

      Show Abstract
      Western Blotting23225259 23225259
      The transmembrane activator TACI triggers immunoglobulin class switching by activating B cells through the adaptor MyD88.
      He, B; Santamaria, R; Xu, W; Cols, M; Chen, K; Puga, I; Shan, M; Xiong, H; Bussel, JB; Chiu, A; Puel, A; Reichenbach, J; Marodi, L; Döffinger, R; Vasconcelos, J; Issekutz, A; Krause, J; Davies, G; Li, X; Grimbacher, B; Plebani, A; Meffre, E; Picard, C; Cunningham-Rundles, C; Casanova, JL; Cerutti, A
      Nature immunology  11  836-45  2010

      Show Abstract
      20676093 20676093
      MyD88 is an adaptor protein in the hToll/IL-1 receptor family signaling pathways.
      Medzhitov, R, et al.
      Mol. Cell, 2: 253-8 (1998)  1998

      Show Abstract
      9734363 9734363
      Targeted disruption of the MyD88 gene results in loss of IL-1- and IL-18-mediated function.
      Adachi, O, et al.
      Immunity, 9: 143-50 (1998)  1998

      9697844 9697844
      IRAK (Pelle) family member IRAK-2 and MyD88 as proximal mediators of IL-1 signaling.
      Muzio, M, et al.
      Science, 278: 1612-5 (1997)  1997

      Show Abstract
      9374458 9374458
      The cloning and characterization of human MyD88: a member of an IL-1 receptor related family.
      Bonnert, T P, et al.
      FEBS Lett., 402: 81-4 (1997)  1997

      9013863 9013863