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06-984 Anti-Mn-SOD Antibody

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06-984
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, BIHC, IH(P), WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-984
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Mn-SOD Antibody
      Alternate Names
      • Mn superoxide dismutase
      • indophenoloxidase B
      • manganese superoxide dismutase
      • manganese-containing superoxide dismutase
      • mangano-superoxide dismutase
      • superoxide dismutase 2, mitochondrial
      Background InformationSuperoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn SOD/SOD1), mitochondrial manganese SOD (Mn SOD/SOD2) and extracellular Cu, Zn SOD (EC SOD/SOD3). The manganese containing 80 kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain.
      References
      Product Information
      FormatPurified
      Control
      • L6 cell lysate, rat brain tissue extract.
      PresentationPurified rabbit polyclonal in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Mn-SOD Antibody is an antibody against Mn-SOD for use in IH, IH(P) & WB.
      Key Applications
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin):
      10 μg/mL of a previous lot detected MnSOD in paraffin embedded rat brain tissue sections.
      Biological Information
      ImmunogenRecombinant human MnSOD (manganous superoxide dismutase).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes MnSOD, Mr 24 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
      Gene Symbol
      • SOD2
      • Mn-SOD
      • MNSOD
      • MnSOD
      • IPO-B
      Non-Reactive Species
      • Canine
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P04179 # Destroys radicals which are normally produced within the cells and which are toxic to biological systems.
      COFACTOR: Binds 1 manganese ion per subunit.
      SIZE: 222 amino acids; 24722 Da
      SUBUNIT: Homotetramer.
      SUBCELLULAR LOCATION: Mitochondrion matrix.SIMILARITY:SwissProt: P04179 ## Belongs to the iron/manganese superoxide dismutase family.
      Molecular Weight~24 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates of MCF-7, A431 or rat L6 RIPA cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected MnSOD in RIPA lysates of rat L6 RIPA cells. 1-4 μg/mL of a previous lot detected MnSOD in RIPA lysates of human MCF-7, A431 and rat L6 RIPA cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2 to 8ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Mn-SOD Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mn-SOD Antibody Certificates of Analysis

      TitleLot Number
      Anti-Mn-SOD 2477947
      Anti-Mn-SOD - 2382159 2382159
      Anti-Mn-SOD - 06-984 06-984
      Anti-Mn-SOD - 18934 18934
      Anti-Mn-SOD - 1970741 1970741
      Anti-Mn-SOD - 1984841 1984841
      Anti-Mn-SOD - 2020543 2020543
      Anti-Mn-SOD - 2087932 2087932
      Anti-Mn-SOD - 2194163 2194163
      Anti-Mn-SOD - 2281662 2281662

      References

      Reference overviewApplicationSpeciesPub Med ID
      Ω3 Supplementation and intermittent hypobaric hypoxia induce cardioprotection enhancing antioxidant mechanisms in adult rats.
      Herrera, EA; Farías, JG; González-Candia, A; Short, SE; Carrasco-Pozo, C; Castillo, RL
      Marine drugs  13  838-60  2015

      Show Abstract
      25658050 25658050
      Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells.
      Hopkins, MM; Feng, X; Liu, M; Parker, LP; Koh, DW
      International journal of oncology  46  2267-76  2015

      Show Abstract
      25760245 25760245
      Tempol treatment reduces anxiety-like behaviors induced by multiple anxiogenic drugs in rats.
      Patki, G; Salvi, A; Liu, H; Atrooz, F; Alkadhi, I; Kelly, M; Salim, S
      PloS one  10  e0117498  2015

      Show Abstract
      25793256 25793256
      Physical activity enhances metabolic fitness independently of cardiorespiratory fitness in marathon runners.
      Laye, MJ; Nielsen, MB; Hansen, LS; Knudsen, T; Pedersen, BK
      Disease markers  2015  806418  2015

      Show Abstract
      Western Blotting25821340 25821340
      Muscle dysfunction associated with adjuvant-induced arthritis is prevented by antioxidant treatment.
      Yamada, T; Abe, M; Lee, J; Tatebayashi, D; Himori, K; Kanzaki, K; Wada, M; Bruton, JD; Westerblad, H; Lanner, JT
      Skeletal muscle  5  20  2015

      Show Abstract
      26161253 26161253
      Daily Oxygen/O₃ Treatment Reduces Muscular Fatigue and Improves Cardiac Performance in Rats Subjected to Prolonged High Intensity Physical Exercise.
      Di Filippo, C; Trotta, MC; Maisto, R; Siniscalco, D; Luongo, M; Mascolo, L; Alfano, R; Accardo, M; Rossi, C; Ferraraccio, F; D'Amico, M
      Oxidative medicine and cellular longevity  2015  190640  2015

      Show Abstract
      26265981 26265981
      Manganese superoxide dismutase expression is negatively associated with microRNA-301a in human pancreatic ductal adenocarcinoma.
      Pandit, H; Zhang, W; Li, Y; Agle, S; Li, X; Li, SP; Cui, G; Li, Y; Martin, RC
      Cancer gene therapy  481-6  2015

      Show Abstract
      Immunohistochemistry26384137 26384137
      IκBβ-mediated NF-κB activation confers protection against hyperoxic lung injury.
      Michaelis, KA; Agboke, F; Liu, T; Han, K; Muthu, M; Galambos, C; Yang, G; Dennery, PA; Wright, CJ
      American journal of respiratory cell and molecular biology  50  429-38  2014

      Show Abstract
      Western Blotting24066808 24066808
      miR-335 correlates with senescence/aging in human mesenchymal stem cells and inhibits their therapeutic actions through inhibition of AP-1 activity.
      Tomé, M; Sepúlveda, JC; Delgado, M; Andrades, JA; Campisi, J; González, MA; Bernad, A
      Stem cells (Dayton, Ohio)  32  2229-44  2014

      Show Abstract
      24648336 24648336
      Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity.
      Alfaradhi, MZ; Fernandez-Twinn, DS; Martin-Gronert, MS; Musial, B; Fowden, A; Ozanne, SE
      American journal of physiology. Regulatory, integrative and comparative physiology  307  R26-34  2014

      Show Abstract
      Western BlottingMouse24789994 24789994

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies