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MAB1273 Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1

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MAB1273
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMAB1273
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1
      Background InformationMitochondria are important cell organelles that are responsible ATP production and biosynthesis of several intermediates. They also play a major role in cellular stress responses, autophagy, and apoptosis. They are also part of a dynamic, interconnected network that is closely integrated with other cellular compartments and their function extends beyond the boundaries of the cell. They affect the physiology of the organism by regulating communication between cells and tissues. Within a cell the mitochondria are in constant motion and can move to the areas of high energy demand and may undergo repeated rounds of fission and fusion, which is important in mixing of mitochondrial matrices and redistribution of the mitochondrial DNA. As a by-product of oxidative phosphorylation, mitochondria produce significant quantities of endogenous reactive oxygen species (ROS), which can damage cellular proteins, lipids, nuclear and mitochondrial DNA. Excessive mitochondrial ROS production can overwhelm the antioxidant defenses and can cause serious damage to mitochondria and the cell. Cumulative oxidative damage and mitochondrial DNA mutations can lead to mitochondrial abnormalities that affect the health of the organism. It has been shown that mitochondria from cancer cells are often resistant to permeabilization of their outer membranes and resist apoptosis. Clone 113-1 is an ideal antibody for staining mitochondria in human cells and can be used as a mitochondrial marker in subcellular fractions. It recognizes a 60 kDa non-glycosylated protein component of mitochondria found in human cells. It is shown to produce a spaghetti-like pattern in normal and malignant cells.
      References
      Product Information
      FormatPurified
      Control
      • COS7 cells.
      PresentationPurified mouse monoclonal IgG1 in buffer containing liquid in PBS. Contains no preservative. Concentrated hybridoma supernatant.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Mitochondria, surface of intact mitochondria, clone 113-1, Cat. No. MAB1273 is a mouse monoclonal antibody that detects Mitochondria and is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected a ~ 60 kDa mitochondrial protein in HepG2 cell lysate.

      Western Blotting Analysis: A representative lot detected a mitochondrial protein in Western Blotting applications (Andreola, G., et al. (2002). J Exp Med.;195(10):1303-16; Dadsena, S., et al. (2019). Nat Commun.10(1):1832).

      Immunocytochemistry Analysis: A representative lot detected mitochondria in Immunocytochemistry applications (Whitlock, B.B., et al. (2000). J Cell Biol. 151(6):1305-20).

      Immunofluorescence Analysis: A representative lot detected Mitochondria in Immunofluorescence applications (Lee, J., et al. (2019). Exp Mol Med. 51(4):1-9; Ko, H.R., et al. (2018). Stem Cell Res Ther.;9(1):326; MacAskill, M.G., et al. (2018). Mol Ther. 26(7):1669-1684)

      Immunohistochemistry Applications: A representative lot detected mitochondria in Immunohistochemistry applications (Ko, H.R., et al. (2018). Stem Cell Res Ther.;9(1):326; MacAskill, M.G., et al. (2018). Mol Ther. 26(7):1669-1684).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      Epitopesurface of intact mitochondria
      Clone113-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityClone 113-1 is a mouse monoclonal antibody that detects a 60 kDa non-glycosylated mitochondrial protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Stem Cell Type
      • Neural Stem Cells
      Molecular Weight65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected a ~60 kDa mitochondrial protein in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Mitochondria Antibody, surface of intact mitochondria, clone 113-1 Certificates of Analysis

      TitleLot Number
      Anti-Mitochondria, surface of intact -2722860 2722860
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 2484154
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2148226 2148226
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2430332 2430332
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 1997360 1997360
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2021176 2021176
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2065960 2065960
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2324503 2324503
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 2493926 2493926
      Anti-Mitochondria, surface of intact mitochondria, clone 113-1 - 3261303 3261303

      References

      Reference overviewApplicationSpeciesPub Med ID
      Homing of neural stem cells from the venous compartment into a brain infarct does not involve conventional interactions with vascular endothelium.
      Goncharova, V; Das, S; Niles, W; Schraufstatter, I; Wong, AK; Povaly, T; Wakeman, D; Miller, L; Snyder, EY; Khaldoyanidi, SK
      Stem cells translational medicine  3  229-40  2014

      Show Abstract
      24396034 24396034
      A Site-Specific Integrated Col2.3GFP Reporter Identifies Osteoblasts Within Mineralized Tissue Formed In Vivo by Human Embryonic Stem Cells.
      Xin, X; Jiang, X; Wang, L; Stover, ML; Zhan, S; Huang, J; Goldberg, AJ; Liu, Y; Kuhn, L; Reichenberger, EJ; Rowe, DW; Lichtler, AC
      Stem cells translational medicine  3  1125-37  2014

      Show Abstract
      25122686 25122686
      Role of mesenchymal stem cells in osteosarcoma and metabolic reprogramming of tumor cells.
      Bonuccelli, G; Avnet, S; Grisendi, G; Salerno, M; Granchi, D; Dominici, M; Kusuzaki, K; Baldini, N
      Oncotarget  5  7575-88  2014

      Show Abstract
      25277190 25277190
      Developmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells.
      Kuhn, LT; Liu, Y; Boyd, NL; Dennis, JE; Jiang, X; Xin, X; Charles, LF; Wang, L; Aguila, HL; Rowe, DW; Lichtler, AC; Goldberg, AJ
      Tissue engineering. Part A  20  365-77  2014

      Show Abstract
      23952622 23952622
      Neural progenitors derived from human induced pluripotent stem cells survive and differentiate upon transplantation into a rat model of amyotrophic lateral sclerosis.
      Popescu, IR; Nicaise, C; Liu, S; Bisch, G; Knippenberg, S; Daubie, V; Bohl, D; Pochet, R
      Stem cells translational medicine  2  167-74  2013

      Show Abstract
      23413376 23413376
      Platelet-rich plasma promotes the proliferation of human muscle derived progenitor cells and maintains their stemness.
      Li, H; Usas, A; Poddar, M; Chen, CW; Thompson, S; Ahani, B; Cummins, J; Lavasani, M; Huard, J
      PloS one  8  e64923  2013

      Show Abstract
      23762264 23762264
      Origin of the vasculature supporting growth of primary patient tumor xenografts.
      Hylander, BL; Punt, N; Tang, H; Hillman, J; Vaughan, M; Bshara, W; Pitoniak, R; Repasky, EA
      Journal of translational medicine  11  110  2013

      Show Abstract
      23639003 23639003
      Functional transplantation of salivary gland cells differentiated from mouse early ES cells in vitro.
      Kawakami, M; Ishikawa, H; Tachibana, T; Tanaka, A; Mataga, I
      Human cell  26  80-90  2013

      Show Abstract
      23681939 23681939
      Magnetic resonance imaging tracking of ferumoxytol-labeled human neural stem cells: studies leading to clinical use.
      Gutova, M; Frank, JA; D'Apuzzo, M; Khankaldyyan, V; Gilchrist, MM; Annala, AJ; Metz, MZ; Abramyants, Y; Herrmann, KA; Ghoda, LY; Najbauer, J; Brown, CE; Blanchard, MS; Lesniak, MS; Kim, SU; Barish, ME; Aboody, KS; Moats, RA
      Stem cells translational medicine  2  766-75  2013

      Show Abstract
      24014682 24014682
      Human prostate cancer in a clinically relevant xenograft mouse model: identification of β(1,6)-branched oligosaccharides as a marker of tumor progression.
      Lange, T; Ullrich, S; Müller, I; Nentwich, MF; Stübke, K; Feldhaus, S; Knies, C; Hellwinkel, OJ; Vessella, RL; Abramjuk, C; Anders, M; Schröder-Schwarz, J; Schlomm, T; Huland, H; Sauter, G; Schumacher, U
      Clinical cancer research : an official journal of the American Association for Cancer Research  18  1364-73  2012

      Show Abstract
      22261809 22261809