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MABE867 Anti-Mad1 Antibody, clone BB3-8

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IFMPurifiedMonoclonal Antibody
      Catalogue NumberMABE867
      DescriptionAnti-Mad1 Antibody, clone BB3-8
      Alternate Names
      • Mitotic spindle assembly checkpoint protein MAD1
      • Mitotic arrest deficient 1-like protein 1
      • MAD1-like protein 1
      • Mitotic checkpoint MAD1 protein homolog
      • HsMAD1
      • hMAD1
      • Tax-binding protein 181
      Background InformationMitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.
      Product Information
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-Mad1 Antibody, clone BB3-8 is a highly specific mouse monoclonal antibody, that targets MAD1 & has been tested in western blotting & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).

      Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).

      Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human Mad1.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAD1L1
      • MAD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~83 kDa observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Mad1 Antibody, clone BB3-8 SDS


      Safety Data Sheet (SDS) 

      Anti-Mad1 Antibody, clone BB3-8 Certificates of Analysis

      TitleLot Number
      Anti-Mad1, clone BB3-8 - 3407289 3407289
      Anti-Mad1, clone BB3-8 - 3589767 3589767
      Anti-Mad1, clone BB3-8 - 3888818 3888818
      Anti-Mad1, clone BB3-8 - Q2239053 Q2239053
      Anti-Mad1, clone BB3-8 -2756663 2756663
      Anti-Mad1, clone BB3-8 -2809945 2809945
      Anti-Mad1, clone BB3-8 -2819600 2819600
      Anti-Mad1, clone BB3-8 Monoclonal Antibody 2931000
      Anti-Mad1, clone BB3-8 Monoclonal Antibody 3022944


      Reference overviewPub Med ID
      Direct binding of Cenp-C to the Mis12 complex joins the inner and outer kinetochore.
      Screpanti, Emanuela, et al.
      Curr. Biol., 21: 391-8 (2011)  2011

      Show Abstract
      21353556 21353556
      Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
      Santaguida, Stefano, et al.
      EMBO J., 30: 1508-19 (2011)  2011

      Show Abstract
      21407176 21407176