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NA27 Anti-MSH2 (Ab-2) Mouse mAb (FE11)

NA27
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M M Monoclonal Antibody

Pricing & Availability

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NA27-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~100 kDa MSH2 protein in HCT116 and SW480 cells and in colon tissue.
      Catalogue NumberNA27
      Brand Family Calbiochem®
      SynonymsAnti-Mismatch Repair Protein 2
      References
      ReferencesBronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals Med. 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlLoVo cells
      Positive controlHCT116 or SW480 cells or colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.
      Key Applications Paraffin Sections
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesFrozen Sections (2 µg/ml, see application references)
      Immunoblotting (0.2 µg/ml, see application references)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena C-terminal fragment of human MSH2
      ImmunogenHuman
      CloneFE11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MSH2 (Ab-2) Mouse mAb (FE11) Certificates of Analysis

      TitleLot Number
      NA27

      References

      Reference overview
      Bronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals Med. 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-September-2007 RFH
      SynonymsAnti-Mismatch Repair Protein 2
      ApplicationFrozen Sections (2 µg/ml, see application references)
      Immunoblotting (0.2 µg/ml, see application references)
      Immunoprecipitation (1 µg/reaction)
      Paraffin Sections (2 µg/ml, heat or proteinase K pre-treatment required, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/O mouse myeloma cells. Recognizes the ~100 kDa MSH2 protein.
      BackgroundThe MSH2 gene is one of at least 4 genes encoding proteins involved in the repair of mismatch nucleotides following DNA replication or repair. Mutations in the MSH2 gene contribute to the development of sporadic colorectal carcinoma, while germline MSH2 mutations are responsible for ~50% of inherited, non-polyposis colorectal carcinoma (HNPCC). MSH2 encodes a protein of 934 amino acids.
      HostMouse
      Immunogen speciesHuman
      Immunogena C-terminal fragment of human MSH2
      CloneFE11
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlHCT116 or SW480 cells or colon tissue
      Negative controlLoVo cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBronner, C.E., et al. 1994. Nature 368, 258.
      Papadopoulos, N., et al. 1994. Science 263, 1625.
      Peltomäki, P.T. 1994. Annals Med. 26, 215.
      Fishel, R., et al. 1993. Cell 75, 1027.
      Leach, F.S., et al. 1993. Cell 75, 1215.
      Lindbolm, A., et al. 1993. Nature Genetics 5, 279.
      Application referencesImmunoblotting, Frozen Sections Leach, F.S., et al. 1996. Cancer Res. 56, 235. Paraffin Sections Thibodeau, S.N., et al. 1996. Cancer Res. 56, 4836.