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MABT171 Anti-MMP-9 (Active) Antibody, clone 4A3

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MABT171
200 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT171
      DescriptionAnti-MMP-9 (Active) Antibody, clone 4A3
      Alternate Names
      • Matrix metalloproteinase-9
      • MMP-9
      • 92 kDa gelatinase
      • 92 kDa type IV collagenase
      • Gelatinase B
      • GELB
      Background InformationMatrix metalloproteinase 9 (MMP-9), a member of the peptidase M10A family, is a multidomain zinc metalloproteinase enzyme, which is highly expressed in neurons and glia of the hippocampus, cerebral cortex, and cerebellum. The active MMP-9 is formed upon cell stimulation by enzymatic cleavage of the precursor, proMMP-9. Because of its function in tissue degradation, MMP-9 is tightly regulated by multiple mechanisms, including glycosylation, inhibition by TIMP1, internalization, and sequestration. MMP-9 plays an important role in long-term potentiation, synaptic plasticity, and memory. Several studies have reported an increase in levels of MMP-9 after CNS injury, as well during the development of various cancers.
      References
      Product Information
      FormatPurified
      Control
      • Active form of MMP-9
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MMP-9 (Active) Antibody, clone 4A3 is validated for use in Western Blotting, IHC for the detection of MMP-9 (Active).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A representative lot from an independent laboratory detected the active form of MMP-9 in WB (Duncan, M. E., et al. 1998. Eur J Biochem. 258(1):37-43).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected the active form of MMP-9 in oesophageal cancer tissues (Duncan, M. E., et al. 1998. Eur J Biochem. 258(1):37-43).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to the N-terminus of human MMP-9 (Active).
      EpitopeN-terminus
      Clone4A3
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of MMP-9 (Active).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MMP9
      • CLG4B
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~67 kDa observed. Under reducing conditions in SDS-PAGE, MMP-9 monomer (proenzyme) is observed as a major band at ~ 92 kDa. Activation of MMP-9 proenzyme with trypsin yields different active forms at ~64, ~67, and ~82 kDa in a time dependent manner (Duncan, M. E., et al. 1998. Eur J Biochem. 258(1):37-43).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot with the active form of MMP-9.

      Western Blot Analysis: A 1:250 dilution from a reprsentative lot detected the active form of MMP-9 in WB.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MMP-9 (Active) Antibody, clone 4A3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MMP-9 (Active) Antibody, clone 4A3 Certificates of Analysis

      TitleLot Number
      Anti-MMP-9 (Active), clone 4A3 - 2465287 2465287
      Anti-MMP-9 (Active), clone 4A3 - 2506524 2506524
      Anti-MMP-9 (Active), clone 4A3 - 2990202 2990202
      Anti-MMP-9 (Active), clone 4A3 - 3257047 3257047
      Anti-MMP-9 (Active), clone 4A3 - Q2055250 Q2055250
      Anti-MMP-9 (Active), clone 4A3 -2673205 2673205
      Anti-MMP-9 (Active), clone 4A3 -2695341 2695341
      Anti-MMP-9 (Active), clone 4A3 -2745868 2745868
      Anti-MMP-9 (Active), clone 4A3 -2779745 2779745
      Anti-MMP-9 (Active), clone 4A3 -2796492 2796492

      References

      Reference overviewPub Med ID
      Human matrix metalloproteinase-9: activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form.
      Duncan, M E, et al.
      Eur. J. Biochem., 258: 37-43 (1998)  1998

      Show Abstract
      9851689 9851689

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies