IM68 Anti-MMP-2 (Ab-8) Mouse mAb (VB3)

IM68
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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IM68-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~72 kDa latent and ~66 kDa active forms of MMP-2 in HUVEC cells and bladder, breast, and ovarian carcinoma tissue.
      Catalogue NumberIM68
      Brand Family Calbiochem®
      SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
      Application Data
      Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-8) Mouse mAb (VB3) (Cat. No. IM68) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F., 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells or bladder, breast, or ovarian carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (1:200-1:400, chemiluminescence)
      Immunofluorescence (4-5 µg/ml, indirect)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogennative human pro-MMP-2
      ImmunogenHuman
      CloneVB3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MMP-2 (Ab-8) Mouse mAb (VB3) Certificates of Analysis

      TitleLot Number
      IM68

      References

      Reference overview
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F., 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-Febuary-2009 RFH
      SynonymsAnti-Gelatinase A, Anti-72 kDa Gelatinase, Anti-Matrix Metalloproteinase 2
      ApplicationImmunoblotting (1:200-1:400, chemiluminescence)
      Immunofluorescence (4-5 µg/ml, indirect)
      Application Data
      Detection of human MMP-2 by immunoblotting. Sample: Human MMP-2. Primary antibody: Anti-MMP-2 (Ab-8) Mouse mAb (VB3) (Cat. No. IM68) (1 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma p3-X63-Ag8.653 cells. Recognizes the ~72 kDa latent and the ~66 kDa active forms of MMP-2.
      BackgroundMatrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMPs share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N-terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in angiogenesis, arthritis, periodontitis, and metastasis. Matrix metalloproteinase-2 (MMP-2) also known by its alternative names of 72 kDa gelatinase/type IV collagenase and gelatinase A (EC 3.4.24.24) cleaves a number of substrates including gelatins, collagen types I, IV, V, VII, X, XI, and XIV, fibronectin, elastin and proteoglycan. MMP-2 is synthesized as a 631 amino acid proenzyme (~72 kDa zymogen) which is proteolytically processed at the first 80 amino acids to the 66 kDa active form. MMP-2 has been identified in a number of tissues, cells, and plasma/serum. It is secreted in a pro-enzyme form (pro-MMP-2) and is activated in vitro by organomercurials (e.g., 4aminophenylmercuric acetate, APMA). Pro-MMP-2 and pro-MMP-2/TIMP-2 complex are specifically activated in vitro by active MT1-MMPs on the surface of tumor cells. Numerous studies have shown a correlation between collagenase expression and metastatic potential and suggest that MMP-2 may be a useful marker for the diagnosis or prognosis of cancer.
      HostMouse
      Immunogen speciesHuman
      Immunogennative human pro-MMP-2
      CloneVB3
      IsotypeIgG₁
      Specieshuman
      Positive controlConditioned medium from dexamethasone-treated HT-1080 or HUVEC cells or bladder, breast, or ovarian carcinoma tissue
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol. 2, 861.
      Stetler-Stevenson, W.G., et al. 1993. FASEB J. 7, 1434.
      Woessner, J.F., 1991. FASEB J. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. In Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.