Millipore Sigma Vibrant Logo

MAB3776 Anti-MDM2 Antibody, clone SMP14

View Products on Sigmaaldrich.com
MAB3776
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited Availability
Limited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3776
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-MDM2 Antibody, clone SMP14
      Alternate Names
      • Murine Double Minute 2
      Background InformationMDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
      References
      Product Information
      FormatPurified
      Control
      • POSITIVE CONTROL: Breast carcinoma
      PresentationLiquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot

      Immunohistochemistry (Frozen and Paraffin-embedded sections)

      Immunoprecipitation

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.
      CloneSMP14
      HostMouse
      SpecificityRecognizes human MDM2.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
      Gene Symbol
      • MDM2
      • hdm2
      • HDMX
      • MGC71221
      • Hdm2
      • HDM2
      • EC 6.3.2.-
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q00987 # Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis.
      SIZE: 491 amino acids; 55233 Da
      SUBUNIT: Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53 and WWOX. Interacts with USP7 and TBRG1. Isoform Mdm2-F does not interact with TP53/p53. Interacts with, and ubiquitinates HIV-1 Tat.
      SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.
      TISSUE SPECIFICITY: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.
      DOMAIN: SwissProt: Q00987 Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the hetero-oligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.
      PTM: Phosphorylated in response to ionizing radiation in an ATM- dependent manner.
      DISEASE: SwissProt: Q00987 # Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.
      SIMILARITY: Belongs to the MDM2/MDM4 family. & Contains 1 RanBP2-type zinc finger. & Contains 1 RING-type zinc finger. & Contains 1 SWIB domain.
      MISCELLANEOUS: MDM2 RING finger mutations that failed to ubiquitinate p53 in vitro did not target p53 for degradation when expressed in cells.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MDM2 Antibody, clone SMP14 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MDM2 Antibody, clone SMP14 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN MDM2 MONOCLONAL ANTIBODY - 2436400 2436400
      MOUSE ANTI-HUMAN MDM2 - 3462093 3462093
      MOUSE ANTI-HUMAN MDM2 - 3698124 3698124
      MOUSE ANTI-HUMAN MDM2 - 3792956 3792956
      MOUSE ANTI-HUMAN MDM2 -2517847 2517847
      MOUSE ANTI-HUMAN MDM2 -2671387 2671387
      MOUSE ANTI-HUMAN MDM2 -2739322 2739322
      MOUSE ANTI-HUMAN MDM2 MONOCLONAL ANTIBODY 2985061
      MOUSE ANTI-HUMAN MDM2 MONOCLONAL ANTIBODY 3135880
      MOUSE ANTI-HUMAN MDM2 MONOCLONAL ANTIBODY 2911971

      References

      Reference overviewPub Med ID
      Replication of minute virus of mice in murine cells is facilitated by virally induced depletion of p21.
      Adeyemi, RO; Pintel, DJ
      Journal of virology  86  8328-32  2012

      Show Abstract
      22623787 22623787
      Cyclin a-CDK phosphorylation regulates MDM2 protein interactions.
      Zhang, T and Prives, C
      J. Biol. Chem., 276: 29702-10 (2001)  2001

      Show Abstract
      11359766 11359766
      Immunochemical analysis of the interaction of p53 with MDM2;--fine mapping of the MDM2 binding site on p53 using synthetic peptides.
      Picksley, S M, et al.
      Oncogene, 9: 2523-9 (1994)  1994

      Show Abstract
      8058315 8058315

      Related Products & Applications

      Related Products

      Catalog Number Description  
      04-1530 Anti-MDM2 Antibody, clone 3G9 Show Pricing & Availability
      MABE281 Anti-MDM2 Antibody, clone 2A10 Show Pricing & Availability
      MABE331 Anti-MDM2 Antibody, clone 4B2C1.11 Show Pricing & Availability
      MABE340 Anti-MDM2 Antibody, clone IF2 Show Pricing & Availability
      MABE340-AF488 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      MABE340-AF555 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 555 Conjugate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies