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MABE340-AF555 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 555 Conjugate

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MABE340-AF555
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue NumberMABE340-AF555
      DescriptionAnti-MDM2 Antibody, clone IF2, Alexa Fluor® 555 Conjugate
      Alternate Names
      • E3 ubiquitin-protein ligase Mdm2
      • Double minute 2 protein
      • Hdm2
      • Oncoprotein Mdm2
      • p53-binding protein Mdm2
      Background InformationE3 ubiquitin-protein ligase Mdm2 (UniProt Q00987; also known as Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2) is encoded by the MDM2 gene (Gene ID 4193) in human. MDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes p53 transcriptional activity by binding to and blocking p53 N-terminal trans-activation domain. MDM2 can also monoubiquitinate p53, thereby promoting p53 cytoplasmic localization to prevent p53 nuclear funtion. In addition, MDM2, when in complex with p300, also mediates p53 polyubiquitination, resulting in p53 proteosomal degradation. MDM2 overexpression is shown to inhibit DNA double-strand break repair mediated through its interaction with Nbs1 in a p53-independent manner.
      References
      Product Information
      FormatAlexaFluor®555
      PresentationPurified mouse monoclonal IgG2b antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 555 Conjugate is validated for use in Immunocytochemistry for the detection of MDM2.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MDM2 in HeLa cells.
      The unconjugated antibody (Cat. Nos. MABE340 & OP46) is shown to be suitable also for immunohistochemistry and Western blotting applications.
      Biological Information
      ImmunogenRecombinant human MDM2.
      EpitopeWithin amino acids 26-169.
      CloneIF2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone IF2 recognizes the ~85/90 kDa (apparent MW) MDM2, as well as isoforms of ~57/60 kDa and ~74/76 kDa by immunoblotting using the unconjugated antibody (Cat. Nos. MABE340 & OP46).
      IsotypeIgG2b
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~85 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HepG2 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected MDM2 in HepG2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies