Millipore Sigma Vibrant Logo

MABE340 Anti-MDM2 Antibody, clone IF2

View Products on Sigmaaldrich.com
MABE340
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE340
      DescriptionAnti-MDM2 Antibody, clone IF2
      Alternate Names
      • E3 ubiquitin-protein ligase Mdm2
      • Double minute 2 protein
      • Hdm2
      • Oncoprotein Mdm2
      • p53-binding protein Mdm2
      Background InformationMDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MDM2 Antibody, clone IF2 is a Mouse Monoclonal Antibody for detection of MDM2 also known as E3 ubiquitin-protein ligase Mdm2 or Hdm2 & has been validated in WB & IHC.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blot Analysis: A representative lot detected MDM2 in Raji, HepG2, and Molt-4 cell lysate.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MDM2 in human prostate cancer tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to human MDM2.
      CloneIF2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~85 kDa and ~60 kDa observed. Uniprot describes many isoforms between 12-56 kDa, which can be highly phosphorylated and ubiquitinated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected MDM2 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MDM2 Antibody, clone IF2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MDM2 Antibody, clone IF2 Certificates of Analysis

      TitleLot Number
      Anti-MDM2, clone IF2 -2786917 2786917
      Anti-MDM2, clone IF2 - 2366584 2366584
      Anti-MDM2, clone IF2 - 3166084 3166084
      Anti-MDM2, clone IF2 - 3205918 3205918
      Anti-MDM2, clone IF2 - 3235641 3235641
      Anti-MDM2, clone IF2 - 3243414 3243414
      Anti-MDM2, clone IF2 - 3273631 3273631
      Anti-MDM2, clone IF2 - 3321075 3321075
      Anti-MDM2, clone IF2 - 3438605 3438605
      Anti-MDM2, clone IF2 - 3490106 3490106

      References

      Reference overviewPub Med ID
      A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus.
      Mayo, L D and Donner, D B
      Proc. Natl. Acad. Sci. U.S.A., 98: 11598-603 (2001)  2001

      Show Abstract
      11504915 11504915

      Related Products & Applications

      Related Products

      Catalog Number Description  
      AP124P Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L Show Pricing & Availability
      04-1530 Anti-MDM2 Antibody, clone 3G9 Show Pricing & Availability
      MAB3776 Anti-MDM2 Antibody, clone SMP14 Show Pricing & Availability
      MABE281 Anti-MDM2 Antibody, clone 2A10 Show Pricing & Availability
      MABE331 Anti-MDM2 Antibody, clone 4B2C1.11 Show Pricing & Availability
      MABE340-AF488 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      MABE340-AF555 Anti-MDM2 Antibody, clone IF2, Alexa Fluor® 555 Conjugate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies