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MABE331 Anti-MDM2 Antibody, clone 4B2C1.11

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MABE331
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE331
      DescriptionAnti-MDM2 Antibody, clone 4B2C1.11
      Alternate Names
      • E3 ubiquitin-protein ligase Mdm2
      • Double minute 2 protein
      • Hdm2
      • Oncoprotein Mdm2
      • p53-binding protein Mdm2
      Background InformationMDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-MDM2 Antibody, clone 4B2C1.11 (mouse monoclonal antibody) validated in WB, IP, ICC to detect MDM2 also known as E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, p53-binding protein Mdm2.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cell lysates (Hjerpe, R., et al. (2010). 42(5):725-735.).

      Immunocytochemistry Analysis: A representative lot from an independent laboratory detected MDM2 in SV5-tagged, fusion p53-EGFP transfected H1299 cells (Hjerpe, R., et al. (2010). 42(5):725-735.).
      Biological Information
      ImmunogenRecombinant protein corresponding to human MDM2.
      Clone4B2C1.11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~55 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: 0.25 µg/mL of this antibody detected MDM2 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MDM2 Antibody, clone 4B2C1.11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MDM2 Antibody, clone 4B2C1.11 Certificates of Analysis

      TitleLot Number
      Anti-MDM2, clone 4B2C1.11 - 2432524 2432524
      Anti-MDM2, clone 4B2C1.11 - 3162526 3162526
      Anti-MDM2, clone 4B2C1.11 - 3310503 3310503
      Anti-MDM2, clone 4B2C1.11 - 3387577 3387577
      Anti-MDM2, clone 4B2C1.11 - Q2017855 Q2017855
      Anti-MDM2, clone 4B2C1.11 -2617692 2617692
      Anti-MDM2, clone 4B2C1.11 -2640028 2640028
      Anti-MDM2, clone 4B2C1.11 -2686155 2686155
      Anti-MDM2, clone 4B2C1.11 -2823965 2823965

      References

      Reference overviewPub Med ID
      Oligomerization conditions Mdm2-mediated efficient p53 polyubiquitylation but not its proteasomal degradation.
      Hjerpe, Roland, et al.
      Int. J. Biochem. Cell Biol., 42: 725-35 (2010)  2010

      Show Abstract
      20080206 20080206

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies