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04-1530 Anti-MDM2 Antibody, clone 3G9

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICC, IHCMPurifiedMonoclonal Antibody
      Catalogue Number04-1530
      DescriptionAnti-MDM2 Antibody, clone 3G9
      Alternate Names
      • Double minute 2 protein
      • Mdm2 p53 binding protein homolog (mouse)
      • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein
      • Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse)
      • Oncoprotein Mdm2
      • double minute 2, human homolog of
      • p53-binding protein
      • mouse double minute 2 homolog
      • mouse double minute 2, human homolog of
      • p53-binding protein
      • p53-binding protein Mdm2
      • ubiquitin-protein ligase E3 Mdm2
      Background InformationMDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumor suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated. SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
      Product Information
      • MCF-7 cell lysate
      PresentationPurified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-MDM2 Antibody, clone 3G9 is a Mouse Monoclonal Antibody for detection of MDM2 also known as Double minute 2 protein or Mdm2 p53 binding protein homolog & has been validated in WB, IP, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory for this application. (Changgong, L., et al. (2002). Molecular and Cellular Biology. 22(21):7562-7571.)
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to human MDM2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes MDM2.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq].
      Gene Symbol
      • MDM2
      • hdm2
      • HDMX
      • Hdm2
      • HDM2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Inhibits TP53/p53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Functions as a ubiquitin ligase E3, in the presence of E1 and E2, toward p53 and itself. Permits the nuclear export of p53 and targets it for proteasome-mediated proteolysis.

      SIZE: 491 amino acids; 55233 Da

      SUBUNIT: Binds p53, p73, ARF(P14), ribosomal protein L5 and specifically to RNA. Can interact also with retinoblastoma protein (RB), E1A-associated protein EP300 and the E2F1 transcription factor. Forms a ternary complex with p53 and WWOX. Interacts with USP7 and TBRG1. Isoform Mdm2-F does not interact with TP53/p53. Interacts with, and ubiquitinates HIV-1 Tat.

      SUBCELLULAR LOCATION: Nucleus, nucleoplasm. Cytoplasm. Nucleus, nucleolus. Note=Expressed predominantly in the nucleoplasm. Interaction with ARF(P14) results in the localization of both proteins to the nucleolus. The nucleolar localization signals in both ARF(P14) and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

      TISSUE SPECIFICITY: Ubiquitous. Isoform Mdm2-A, isoform Mdm2-B, isoform Mdm2-C, isoform Mdm2-D, isoform Mdm2-E, isoform Mdm2-F and isoform Mdm2-G are observed in a range of cancers but absent in normal tissues.

      DOMAIN: Region I is sufficient for binding p53 and inhibiting its G1 arrest and apoptosis functions. It also binds p73 and E2F1. Region II contains most of a central acidic region required for interaction with ribosomal protein L5 and a putative C4-type zinc finger. The RING finger domain which coordinates two molecules of zinc interacts specifically with RNA whether or not zinc is present and mediates the hetero-oligomerization with MDM4. It is also essential for its ubiquitin ligase E3 activity toward p53 and itself.

      PTM: Phosphorylated in response to ionizing radiation in an ATM- dependent manner.

      DISEASE: Seems to be amplified in certain tumors (including soft tissue sarcomas, osteosarcomas and gliomas). A higher frequency of splice variants lacking p53 binding domain sequences was found in late-stage and high-grade ovarian and bladder carcinomas. Four of the splice variants show loss of p53 binding.

      SIMILARITY: Belongs to the MDM2/MDM4 family. & Contains 1 RanBP2-type zinc finger. & Contains 1 RING-type zinc finger. & Contains 1 SWIB domain.

      MISCELLANEOUS: MDM2 RING finger mutations that failed to ubiquitinate p53 in vitro did not target p53 for degradation when expressed in cells.
      Molecular Weight ~ 75 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in MCF-7 cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected MDM2 on 10 µg of MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-MDM2 Antibody, clone 3G9 SDS


      Safety Data Sheet (SDS) 

      Anti-MDM2 Antibody, clone 3G9 Certificates of Analysis

      TitleLot Number
      Anti-MDM2, clone 3G9 Monoclonal Antibody 2931196
      Anti-MDM2, clone 3G9 (mouse monoclonal) Monoclonal Antibody Q2922304
      Anti-MDM2, clone 3G9 - 2147133 2147133
      Anti-MDM2, clone 3G9 - 2430474 2430474
      Anti-MDM2, clone 3G9 - 1975102 1975102
      Anti-MDM2, clone 3G9 - 2110567 2110567
      Anti-MDM2, clone 3G9 - 2294367 2294367
      Anti-MDM2, clone 3G9 - 2510206 2510206
      Anti-MDM2, clone 3G9 - 2530008 2530008
      Anti-MDM2, clone 3G9 - 3187382 3187382


      Reference overviewPub Med ID
      STAT3 regulated ARF expression suppresses prostate cancer metastasis.
      Pencik, J; Schlederer, M; Gruber, W; Unger, C; Walker, SM; Chalaris, A; Marié, IJ; Hassler, MR; Javaheri, T; Aksoy, O; Blayney, JK; Prutsch, N; Skucha, A; Herac, M; Krämer, OH; Mazal, P; Grebien, F; Egger, G; Poli, V; Mikulits, W; Eferl, R; Esterbauer, H; Kennedy, R; Fend, F; Scharpf, M; Braun, M; Perner, S; Levy, DE; Malcolm, T; Turner, SD; Haitel, A; Susani, M; Moazzami, A; Rose-John, S; Aberger, F; Merkel, O; Moriggl, R; Culig, Z; Dolznig, H; Kenner, L
      Nature communications  6  7736  2015

      Show Abstract
      26198641 26198641
      Transcriptional repressor NIR interacts with the p53-inhibiting ubiquitin ligase MDM2.
      Heyne, K; Förster, J; Schüle, R; Roemer, K
      Nucleic acids research  42  3565-79  2014

      Show Abstract
      24413661 24413661
      A novel mechanism of crosstalk between the p53 and NFκB pathways: MDM2 binds and inhibits p65RelA.
      Heyne, K; Winter, C; Gerten, F; Schmidt, C; Roemer, K
      Cell cycle (Georgetown, Tex.)  12  2479-92  2013

      Show Abstract
      23839035 23839035

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      Life Science Research > Antibodies and Assays > Primary Antibodies