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MABE281 Anti-MDM2 Antibody, clone 2A10

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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Catalogue NumberMABE281
      DescriptionAnti-MDM2 Antibody, clone 2A10
      Alternate Names
      • E3 ubiquitin-protein ligase Mdm2
      • Double minute 2 protein
      • Hdm2
      • Oncoprotein Mdm2
      • p53-binding protein Mdm2
      Background InformationMDM2 is an E3 ubiquitin ligase that is most widely studied for its role in regulating the p53 tumor suppressor. MDM2 antagonizes the transcriptional activity of p53 in two main ways. The N-terminal of MDM2 interacts and disables the transactivation domain of p53. In addition, the C-terminal E3 ligase region of MDM2 can attach monoubiquitin to p53 resulting in the transport of p53 from the nucleus to the cytoplasm, thereby preventing the interaction of p53 with target DNA sequences. In addition, MDM2 may also attach polyubiquitin chains to p53, resulting in the degradation of p53 by the proteosome. MDM2 may produce these effects in cooperation with the Mdmx protein. Overall, MDM2 inhibits the ability of p53 to induce cell cycle arrest or apoptosis, and may contribute to oncogenesis.
      Product Information
      • A-549 cell lysate
      PresentationPurified mouse monoclonal IgG2aκ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationAnti-MDM2 Antibody, clone 2A10 is a Mouse Monoclonal Antibody for detection of MDM2 also known as E3 ubiquitin-protein ligase Mdm2 or Hdm2 & has been validated in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot was used by an independent laboratory in human lymphoblastoid cell line (NL553) treated with NCS. (Khosravi, R., et al. (1999). PNAS. 96(26):14973–14977.)
      Biological Information
      ImmunogenHuman MDM2 protein
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MDM2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~90 kDa observed. The calculated molecular weight is 55 kDa, however MDM2 has been shown as a ~90 kDa band in western blots (Erhardt, P., et al. (1997). The Journal of Biological Chemistry. 272:15049-15052.). Uniprot describes many isoforms between 12-56 kDa, which can be highly phosphorylated and ubiquitinated.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A-549 cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected MDM2 on 10 µg of A-549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-MDM2 Antibody, clone 2A10 SDS


      Safety Data Sheet (SDS) 

      Anti-MDM2 Antibody, clone 2A10 Certificates of Analysis

      TitleLot Number
      Anti-MDM2, clone 2A10 - 2225203 2225203
      Anti-MDM2, clone 2A10 - 3182612 3182612
      Anti-MDM2, clone 2A10 - 3186114 3186114
      Anti-MDM2, clone 2A10 - 3275067 3275067
      Anti-MDM2, clone 2A10 - 3281614 3281614
      Anti-MDM2, clone 2A10 - 3385846 3385846
      Anti-MDM2, clone 2A10 - 3574555 3574555
      Anti-MDM2, clone 2A10 -2540033 2540033
      Anti-MDM2, clone 2A10 -2557352 2557352
      Anti-MDM2, clone 2A10 -2671387 2671387


      Reference overviewPub Med ID
      Rapid ATM-dependent phosphorylation of MDM2 precedes p53 accumulation in response to DNA damage.
      Khosravi, R, et al.
      Proc. Natl. Acad. Sci. U.S.A., 96: 14973-7 (1999)  1999

      Show Abstract
      10611322 10611322
      Identification of the MDM2 oncoprotein as a substrate for CPP32-like apoptotic proteases.
      Erhardt, P, et al.
      J. Biol. Chem., 272: 15049-52 (1997)  1997

      Show Abstract
      9182520 9182520

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      Life Science Research > Antibodies and Assays > Primary Antibodies