OP145 Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

Pricing & Availability

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OP145-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.
      Catalogue NumberOP145
      Brand Family Calbiochem®
      SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
      Application Data
      Detection of human MDM2 by immunocytochemistry. Sample: MCF7 cells. Primary antibody: Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) (Cat. No. OP145) (2.5 µg/ml). Detection: fluorescence.
      References
      ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
      Positive controlA549 or MCF7 cells or breast carcinoma tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.
      Key Applications Paraffin Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (2 µg/ml, chemiluminescence)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/reaction, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, human MDM2
      ImmunogenHuman
      Clone4B2C1.11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) Certificates of Analysis

      TitleLot Number
      OP145

      References

      Reference overview
      Marchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-June-2011 RFH
      SynonymsAnti-Ubiquitin Protein Ligase, Anti-p53 Binding Protein, Ant-Murine Double Minute Chromosome-2
      ApplicationImmunoblotting (2 µg/ml, chemiluminescence)
      Immunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/reaction, see application references)
      Paraffin Sections (2.5 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human MDM2 by immunocytochemistry. Sample: MCF7 cells. Primary antibody: Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) (Cat. No. OP145) (2.5 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.
      BackgroundIncreased expression of proto-oncogenes due to gene amplification can lead to cellular transformation in the absence of other mutagenic events. Recently, a novel gene termed MDM2, first identified in mouse as a small acentromeric extrachromosomal element, was shown to become oncogenic upon amplification and tumorigenic when overexpressed in NIH3T3 and Rat2 cells. The human homolog of MDM2 gene has been isolated and mapped to chromosome 12q13-q14, a region that is found to be amplified in human sarcomas. Sequence analysis of the murine, rat, and human MDM2 genes suggests that MDM2 may be involved in transcriptional regulation. MDM2 contains two putative metal-binding motifs, one of which shares similarity to known zinc finger transcriptional activators. MDM2 also contains a putative nuclear localization signal and an acidic domain of the type found in transcriptional activators. A functional role for MDM2 has recently been identified on the basis of experiments which demonstrate that MDM2 forms a stable complex with p53 in vivo. Furthermore, over-expression of MDM2 inhibits the ability of wild type p53 to stimulate expression of target genes and this inhibition appears to result from MDM2 binding to the acidic activation domain of p53, thereby preventing p53 from directly contacting the transcriptional machinery. Interestingly, wild type p53 appears to positively regulate expression of the MDM2 gene. MDM2 amplification has been observed in sarcomas and direct analysis of the MDM2 and p53 genes in sarcomas indicates that one or the other but not both of these genes is mutated in 70% of the tumors. Thus, it appears that genetic alterations in either p53 or MDM2 represent alternative mechanisms for inactivating the same growth suppressive pathway. Regulation of MDM2 expression by p53 also represents a potential feed back control of p53 function.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant, human MDM2
      Clone4B2C1.11
      IsotypeIgG₁
      Specieshuman
      Positive controlA549 or MCF7 cells or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMarchetti, A., et al. 1995. J. Pathol. 175, 31.
      Barak, Y., et al. 1993. EMBO. J. 12, 461.
      Ladanyi, M., et al. 1993. Cancer Res. 53, 16.
      Leach, F.S., et al. 1993. Cancer Res. 53, 2231.
      Oliner, J.D., et al. 1993. Nature 362, 857.
      Momand, J., et al. 1992. Cell 69, 1237.
      Oliner, J.D., et al. 1992. Nature 358, 80.
      Fakharzadeh, S.S., et al. 1991. EMBO J. 10, 1565.
      Application referencesImmunoprecipitation Chen, J., et al. 1993. Mol. Cell Biol. 13, 4107.