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MABF2316-100UL Anti-MCPyV T-antigen Antibody, clone 2t2

MABF2316-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2316-100UL
      DescriptionAnti-MCPyV T-antigen Antibody, clone 2t2
      Alternate Names
      • Merkel cell polyomavirus T antigen
      • MCV Large T antigen
      Background InformationMerkel cells are neuroendocrine cells found in skin that have synaptic contacts with somatosensory afferent terminals. Merkel cell carcinoma (MCC), a rare, but deadly neuroendocrine skin tumor that is caused by clonal integration of a Merkel cell polyomavirus (MCPyV) into these cells. Human polyomavirus is small nonenveloped double-stranded DNA viruses with their genome divided into early, late, and noncoding control regions. The early region encodes for large T (LT) and small T (sT) regulatory proteins and the late region comprises structural genes that produce viral capsid proteins VP1, VP2, and VP3. MCPyV-associated tumorigenesis is characterized and mediated by the sole expression of LT and sT antigens. The LT antigens contain several common motifs and domains that are shown to be important for facilitating the viral life cycle. The N-terminal end of LT (aa 1 70 aa) contains the DnaJ domain that consists of the CR1 (aa 13-17) motif followed by the HPDKGG hexapeptide sequence responsible for Hsc70 binding. The C-terminal portion of LT contains several critical elements that are essential for viral replication. It is reported that the T-antigen origin binding domain (OBD; aa 308-433) of LT is responsible for recognition and binding of a minimum 71-bp origin of replication in the noncoding regulatory region. It also contains a zinc-finger motif (aa 437-528) with in the core polyomavirus helicase/ ATPase domain that is shown to be essential for LT oligomerization and initiation of replication. LT of MCPyV also has a short nuclear localization sequence (aa 277-280). (Ref.: Wendzicki , JA et al., (2015). Curr. Opin. Virol. 11: 38-43; Verhaegen, ME et al., (2015). J. Invest. Dermatol. 135(5); 1415-1424; Toptan, T., et al. (2016). J. Clin. Invest. Insight. 1(2). pii: e85562).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-MCPyV T-antigen, clone 2t2, Cat. No. MABF2316, is a mouse monoclonal antibody that detects Truncated large T antigen and has been tested for use in Immunocytochemistry, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunohistochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).

      Western Blotting Analysis: A representative lot detected MCPyV T-antigen in Western Blotting applications (Wang, X., et. al. (2012). PLoS Pathog. 8(11); Shuda, M., et. al. (2014). J Invest Dermatol. 134(5):1479-1481; Toptan, T., et. al. (2016). JCI Insight. 1(2)).

      Immunocytochemistry Analysis: A representative lot detected MCPyV T-antigen in Immunocytochemistry applications (Toptan, T., et. al. (2016). JCI Insight. 1(2)).
      Biological Information
      ImmunogenA linear peptide corresponding to 17 amino acids from the J-domain region present in both MCV LT and sT proteins.
      Clone2t2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2t2 is a mouse monoclonal antibody that detects MCV LT and the 57 kDa T.
      IsotypeIgG1κ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus transformed human cells.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~17 and 48 kDa observed; 28.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MKL-1 cell lysates.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MCPyV T-antigen in MKL-1 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MCPyV T-antigen Antibody, clone 2t2 Certificates of Analysis

      TitleLot Number
      Anti-MCPyV T-antigen, clone 2t2 - 35009483500948
      Anti-MCPyV T-antigen, clone 2t2 - Q3238223Q3238223

      References

      Reference overviewPub Med ID
      Survey for human polyomaviruses in cancer.
      Toptan, T; Yousem, SA; Ho, J; Matsushima, Y; Stabile, LP; Fernández-Figueras, MT; Bhargava, R; Ryo, A; Moore, PS; Chang, Y
      JCI Insight  1  2016

      Show Abstract
      27034991 27034991
      Merkel cell polyomavirus-positive Merkel cell carcinoma requires viral small T-antigen for cell proliferation.
      Shuda, M; Chang, Y; Moore, PS
      J Invest Dermatol  134  1479-1481  2014

      Show Abstract
      24217011 24217011
      Bromodomain protein Brd4 plays a key role in Merkel cell polyomavirus DNA replication.
      Wang, X; Li, J; Schowalter, RM; Jiao, J; Buck, CB; You, J
      PLoS Pathog  8  e1003021  2012

      Show Abstract
      23144621 23144621