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ST1641 Anti-MAP4K4 Mouse mAb (4A5)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H M Monoclonal Antibody

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ST1641-100UG
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      100 μg
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      Description
      OverviewRecognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.
      Catalogue NumberST1641
      Brand Family Calbiochem®
      SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
      Application Data
      Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlK-562 cells, Human breast cancer tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
      Clone4A5
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-MAP4K4 Mouse mAb (4A5) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-November-2010 RFH
      SynonymsAnti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 4
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human MAP4K4 by immunoblotitng. Sample: Whole cell lysate from K-562 cells. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MAP4K4 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human breast cancer tissue. Primary antibody: Anti-MAP4K4 Mouse mAb (4A5) (Cat. No. ST1641) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.
      BackgroundThe protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-α signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein
      Clone4A5
      IsotypeIgG₁
      Specieshuman
      Positive controlK-562 cells, Human breast cancer tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review