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06-583 Anti-Lck Antibody

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06-583
200 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-583
      Replaces04-372
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Lck Antibody
      Background InformationTyrosine-protein kinase Lck (UniProt: P06239; also known a EC:2.7.10.2, Leukocyte C-terminal Src kinase, LSK, Lymphocyte cell-specific protein-tyrosine kinase, Protein YT16, Proto-oncogene Lck, T cell-specific protein-tyrosine kinase, p56-LCK) is encoded by the LCK gene (Gene ID: 3932) in human. Lck is a non-receptor tyrosine kinase that is specifically expressed in lymphoid cells. Its protein kinase domain is localized to amino acids 245-498. It plays an essential role in the selection and maturation of developing T-cells in the thymus and also plays a role in function of mature T-cells. It is reported to constitutively associate with the cytoplasmic region of the CD4 and CD8 receptors. It also plays a role in T-cell antigen receptor (TCR)-linked signaling. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively. This results in recruitment of Lck to the vicinity of the TCR/CD3 complex where Lck can phosphorylate tyrosine residues within the immunoreceptor tyrosine-based activation motifs (ITAM) of the cytoplasmic tails of the TCR- chains and CD3 subunits that initiates the TCR/CD3 signaling. Once stimulated, the TCR recruits the tyrosine kinase ZAP70 that becomes phosphorylated and activated by Lck. Following this, a large number of signaling molecules are recruited, ultimately leading to lymphokine production. Autophosphorylation of Lck on tyrosine 394 increases its enzyme activity, whereas phosphorylation on tyrosine 505 by C-terminal Src kinase (CSK) results in a reduction in its activity. (Ref.: Rossy, J., et al. (2012). Front. Immunol. 3; 167; Bergman, M., et al. (1992). EMBO J. 11(8); 2919-2924).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Lck , Cat. No. 06-583, is a rabbit polyclonal antibody that detects Lck and is tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesTested Applications

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Lck in Jurkat cell lysate.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 58 amino acids from the N-terminal region of human Tyrosine-protein kinase Lck.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Tyrosine-protein kinase Lck. It targets an epitope within the N-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Bovine.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein is a key signaling molecule in the selection and maturation of developing T-cells. It contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to the plasma membrane and pericentrosomal vesicles, and binds to cell surface receptors, including CD4 and CD8, and other signaling molecules. Multiple alternatively spliced variants, encoding the same protein, have been described.
      Gene Symbol
      • LCK
      • p56lck
      • YT16
      • pp58lck
      • p56-LCK
      • LSK
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyperphosphorylation and activation. Also plays a role in the IL2 receptor-linked signaling pathway that controls T-cell proliferative response. Binding of IL2 to its receptor results in increased activity of LCK. Is expressed at all stages of thymocyte development and is required for the regulation of maturation events that are governed by both pre-TCR and mature alpha beta TCR.


      SIZE: 509 amino acids; 58001 Da

      SUBUNIT: Binds to the cytoplasmic domain of cell surface receptors, such as CD2, CD4, CD5, CD8, CD44, CD45 and CD122. Also binds to effector molecules, such as PI4K, VAV1, RASA1, FYB and to other protein kinases including CDC2, RAF1, ZAP70 and SYK. Binds to phosphatidylinositol 3'-kinase (PI3K) from T-lymphocytes through its SH3 domain and to the tyrosine phosphorylated form of KHDRBS1/p70 through its SH2 domain. Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine-kinase activity. Interacts with SQSTM1. Interacts with phosphorylated LIME1. Interacts with CBLB.

      SUBCELLULAR LOCATION: Cytoplasm. Cell membrane; Lipid-anchor; Cytoplasmic side. Note=Present in lipid rafts in an unactive form.

      TISSUE SPECIFICITY: Expressed specifically in lymphoid cells.


      DOMAIN: SwissProt: P06239 The SH2 domain mediates interaction with SQSTM1. Interaction is regulated by Ser-59 phosphorylation.

      PTM: Phosphorylated on Tyr-394, which increases enzymatic activity (By similarity). Phosphorylated on Tyr-505, which decreases activity.
      DISEASE:SwissProt: P06239 # A chromosomal aberration involving LCK is found in leukemias. Translocation t(1;7)(p34;q34) with TCRB.

      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
      Molecular Weight~56 kDa observed; 58.0 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lck in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      06-583 04053252731907

      Documentation

      Anti-Lck Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Lck Antibody Certificates of Analysis

      TitleLot Number
      Anti-Lck (rabbit polyclonal IgG) 3098914
      Anti-Lck - 15799 15799
      Anti-Lck - 18782 18782
      Anti-Lck - 25168 25168
      Anti-Lck - 32077 32077
      Anti-Lck - 3224212 3224212
      Anti-Lck - 3457759 3457759
      Anti-Lck - 3989528 3989528
      Anti-Lck -2733570 2733570
      Anti-Lck Polyclonal Antibody Q2922320

      References

      Reference overviewApplicationPub Med ID
      The kinase inhibitor dasatinib induces apoptosis in chronic lymphocytic leukemia cells in vitro with preference for a subgroup of patients with unmutated IgVH genes.
      Veldurthy, Aditya, et al.
      Blood, 112: 1443-52 (2008)  2008

      18550857 18550857
      Revaluation of the role of cholesterol in stabilizing rafts implicated in T cell receptor signaling.
      Alexandre K Rouquette-Jazdanian,Claudette Pelassy,Jean-Philippe Breittmayer,Claude Aussel
      Cellular signalling  18  2006

      Show Abstract
      15925486 15925486
      Phase I pharmacokinetic and pharmacodynamic study of 17-allylamino, 17-demethoxygeldanamycin in patients with advanced malignancies.
      Banerji, U; O'Donnell, A; Scurr, M; Pacey, S; Stapleton, S; Asad, Y; Simmons, L; Maloney, A; Raynaud, F; Campbell, M; Walton, M; Lakhani, S; Kaye, S; Workman, P; Judson, I
      Journal of clinical oncology : official journal of the American Society of Clinical Oncology  23  4152-61  2005

      Show Abstract
      15961763 15961763
      Pharmacokinetic-pharmacodynamic relationships for the heat shock protein 90 molecular chaperone inhibitor 17-allylamino, 17-demethoxygeldanamycin in human ovarian cancer xenograft models.
      Banerji, U; Walton, M; Raynaud, F; Grimshaw, R; Kelland, L; Valenti, M; Judson, I; Workman, P
      Clinical cancer research : an official journal of the American Association for Cancer Research  11  7023-32  2005

      Show Abstract
      16203796 16203796
      Volume expansion stimulates p72(syk) and p56(lyn) in skate erythrocytes.
      Musch, M W, et al.
      J. Biol. Chem., 274: 7923-8 (1999)  1999

      Show Abstract
      Kinase Assay10075687 10075687
      Association of p59(fyn) with the T lymphocyte costimulatory receptor CD2. Binding of the Fyn Src homology (SH) 3 domain is regulated by the Fyn SH2 domain
      Lin, H., et al
      J Biol Chem, 273:19914-21 (1998)  1998

      Immunoprecipitation9677430 9677430
      Changes in expression of signal transduction proteins in T lymphocytes of patients with leprosy
      Zea, A. H., et al
      Infect Immun, 66:499-504 (1998)  1998

      Immunoblotting (Western)9453602 9453602
      Zinc is essential for binding of p56(lck) to CD4 and CD8alpha.
      Lin, R S, et al.
      J. Biol. Chem., 273: 32878-82 (1998)  1998

      Show Abstract
      Immunoprecipitation, Immunoblotting (Western)9830036 9830036
      Signaling through intercellular adhesion molecule 1 (ICAM-1) in a B cell lymphoma line. The activation of Lyn tyrosine kinase and the mitogen-activated protein kinase pathway
      Holland, J. and Owens, T.
      J Biol Chem, 272:9108-12 (1997)  1997

      Immunoprecipitation9083038 9083038
      Pattern of expression of five alternative transcripts of the lck gene in different hematopoietic malignancies: correlation of the level of lck messenger RNA I B with the immature phenotype of the malignancy.
      Rouer, E, et al.
      Cell Growth Differ., 5: 659-66 (1994)  1994

      Show Abstract
      8086341 8086341

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies