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MABS1771-100UL Anti-LGR4 Antibody, clone 5A3

MABS1771-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS1771-100UL
      DescriptionAnti-LGR4 Antibody, clone 5A3
      Alternate Names
      • Leucine-rich repeat-containing G-protein coupled receptor 4
      • G-protein coupled receptor 4
      Background InformationLeucine-rich repeat-containing G-protein coupled receptor 4 (UniProt: Q9BXB1; also known as G-protein coupled receptor 48, LGR4) is encoded by the LGR4 (also known as GPR48) gene (Gene ID: 55366) in human. LGR4 is a multi-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. LGR4 contains fifteen LRR repeats. It is expressed in multiple steroidogenic tissues. Its expression has been reported in basal and granular cells in the skin, ductal epithelial cells in the breast, spermatogonia and tubule cells in the testis, islet cells in the pancreas, and tumor cells in the majority of colon cancer cases. LGR4 functions mainly through Wnt/β-catenin signaling to regulate cell proliferation, differentiation, and adult stem cell homeostasis. It serves as a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins, it is shown to associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors. This triggers the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, it does not activate heterotrimeric G-proteins to transduce the signal. LGR4 also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. It is also implicated in the survival of stem cells in the gut. Haplotype insufficiency of LGR4 is linked to increased risk of squamous cell carcinoma of the skin and to reduced birth weight and electrolyte imbalance. Mutations in LGR4 gene are known to cause osteoporosis. Clone 5A3 is reported to detect LGR4 in human and mouse tissues. (Ref.: Yi, J., et al. (2013). PLoS ONE. 8(10); e78144; Li, Z., et al. 2015). Front. Endocrinol. 6; 131).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LGR4, clone 5A3, Cat. No. MABS1771, is a rat monoclonal antibody that detects Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) and is tested for use in Immunohistochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Applications: A representative lot detected LGR4 in Immunohistochemistry applications (Yi, J., et. al. (2013). PLoS One. 8(10):e78144).

      Western Blotting Analysis: A representative lot detected LGR4 in Western Blotting applications (Yi, J., et. al. (2013). PLoS One. 8(10):e78144).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull length human LGR4 in an expression vector.
      EpitopeUnknown
      Clone5A3
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 5A3 is a rat monoclonal antibody that detects Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LGR4
      • GPR48
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~130 kDa observed; 104.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells transfected with human LGR4.

      Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected Leucine-rich repeat-containing G-protein coupled receptor 4 (LGR4) in lysate from HEK293T cells transfected with HA tagged human LGR4.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications