CBL218 Anti-Keratin K3/K76 Antibody, clone AE5

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CBL218
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Rb WB, IHC, IH(P) M Purified Monoclonal Antibody
      Description
      Catalogue NumberCBL218
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Keratin K3/K76 Antibody, clone AE5
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry on frozen sections: 1:50.

      Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

      Western blotting: 1:500.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRabbit corneal epithelial keratin
      CloneAE5
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
      Gene Symbol
      • KRT3
      • CK3
      • K3
      • CK-3
      • Cytokeratin-3
      • Keratin-3
      Purification MethodIon-exchange chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q01546 # Probably contributes to terminal cornification.
      SIZE: 638 amino acids; 65871 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins.
      DEVELOPMENTAL STAGE: Synthesized during maturation of epidermal keratinocytes.
      SIMILARITY: SwissProt: Q01546 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Molecular Weight64 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Keratin K3/K76 Antibody, clone AE5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Keratin K3/K76 Antibody, clone AE5 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-KERATIN K3/K76 - 24832082483208
      MOUSE ANTI-KERATIN K3/K76 - 25124132512413
      MOUSE ANTI-KERATIN K3/K76 - 31593303159330
      MOUSE ANTI-KERATIN K3/K76 - 31849923184992
      MOUSE ANTI-KERATIN K3/K76 - 32024003202400
      MOUSE ANTI-KERATIN K3/K76 - 32440433244043
      MOUSE ANTI-KERATIN K3/K76 - 32771643277164
      MOUSE ANTI-KERATIN K3/K76 - 33184173318417
      MOUSE ANTI-KERATIN K3/K76 - 33876093387609
      MOUSE ANTI-KERATIN K3/K76 - 34659573465957

      References

      Reference overviewPub Med ID
      Results of a phase I/II clinical trial: standardized, non-xenogenic, cultivated limbal stem cell transplantation.
      Zakaria, N; Possemiers, T; Dhubhghaill, SN; Leysen, I; Rozema, J; Koppen, C; Timmermans, JP; Berneman, Z; Tassignon, MJ
      Journal of translational medicine  12  58  2014

      Show Abstract
      24589151 24589151
      A small molecule inhibitor of SRC family kinases promotes simple epithelial differentiation of human pluripotent stem cells.
      Lian, X; Selekman, J; Bao, X; Hsiao, C; Zhu, K; Palecek, SP
      PloS one  8  e60016  2013

      Show Abstract
      23527294 23527294
      Carrier-free epithelial cell sheets prepared by enzymatic degradation of collagen gel.
      Qicheng Ke,Xiaoran Wang,Qianying Gao,Zheng Wu,Pengxia Wan,Weijiao Zhan,Jian Ge,Zhichong Wang
      Journal of tissue engineering and regenerative medicine  5  2011

      Show Abstract
      20603893 20603893
      Isolation, culture, characterization and optimization of human corneal stem cells.
      Sharifi, AM; Darabi, R; Jadidi, K
      Biocell : official journal of the Sociedades Latinoamericanas de Microscopía Electronica ... et. al  34  53-5  2010

      Show Abstract
      20506631 20506631
      A biomimetic scaffold for culturing limbal stem cells: a promising alternative for clinical transplantation.
      Subhadra Dravida, Subhash Gaddipati, May Griffith, Kim Merrett, Soundarya Lakshmi Madhira, Virender S Sangwan, Geeta K Vemuganti, Subhadra Dravida, Subhash Gaddipati, May Griffith, Kim Merrett, Soundarya Lakshmi Madhira, Virender S Sangwan, Geeta K Vemuganti
      Journal of tissue engineering and regenerative medicine  2  263-71  2008

      Show Abstract
      18512269 18512269
      Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure.
      Kawakita, T; Espana, EM; He, H; Li, W; Liu, CY; Tseng, SC
      The American journal of pathology  167  381-93  2005

      Show Abstract
      16049325 16049325
      Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane.
      Wang, DY; Hsueh, YJ; Yang, VC; Chen, JK
      Investigative ophthalmology & visual science  44  4698-704  2003

      Show Abstract
      14578389 14578389
      Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression.
      Cooper, D; Sun, TT
      The Journal of biological chemistry  261  4646-54  1986

      Show Abstract
      2420789 2420789
      Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
      Schermer, A; Galvin, S; Sun, TT
      The Journal of cell biology  103  49-62  1986

      Show Abstract
      2424919 2424919
      Monoclonal antibody analysis of bovine epithelial keratins. Specific pairs as defined by coexpression.
      Cooper, D and Sun, T T
      J. Biol. Chem., 261: 4646-54 (1986)  1986

      2420789 2420789

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      Life Science Research > Antibodies and Assays > Primary Antibodies