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MAB1961Z Anti-Integrin αVβ5 Antibody, clone P1F6, azide free

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MAB1961Z
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, HFC, IHC, FUNCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB1961Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin αVβ5 Antibody, clone P1F6, azide free
      Alternate Names
      • MAB1961
      Background InformationThe product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha V chain. Alpha chain V undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling.
      References
      Product Information
      FormatPurified
      Control
      • Epithelium, keratinocytes
      PresentationPurified immunoglobulin in 0.01M phosphate buffer, pH 7.1, 0.15M NaCl containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect Integrin αVβ5 using this Anti-Integrin αVβ5 Antibody, clone P1F6, azide free validated for use in FC, IH.
      Key Applications
      • Flow Cytometry
      • Immunohistochemistry
      • Affects Function
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesInhibition of attachment of HT29 cells to vitronectin: >1:1,000

      Inhibition of attachment of carcinoma and melanoma cells to vitronectin

      Immunohistochemistry: for use on acetone or paraformaldehyde fixed tissue. (Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.)

      Immunocytochemistry: for use on human carcinomas, normal epithelium and keratinocytes 1:1500 (Acetone Fixed Only)

      Immunoprecipitation

      FACS

      EIA

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman UCLA P3 cells (lung carcinoma)
      CloneP1F6
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with the human integrin alphaVbeta5.
      IsotypeIgG1
      Species Reactivity
      • Chicken
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      MAB1961Z 04053252501524

      Documentation

      Anti-Integrin αVβ5 Antibody, clone P1F6, azide free SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin αVβ5 Antibody, clone P1F6, azide free Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN #945;V#946;5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2074048 2074048
      MOUSE ANTI-HUMAN INTEGRIN aVB5 PRESERVATIVE FREE MONOCLONAL ANTIBODY 2918939
      MOUSE ANTI-HUMAN INTEGRIN aVb5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2289144 2289144
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2428686 2428686
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 PRESERVATIVE FREE MONOCLONAL ANTIBODY - 2465271 2465271
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3237513 3237513
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3433648 3433648
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3683816 3683816
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3774201 3774201
      MOUSE ANTI-HUMAN INTEGRIN αVβ5 - 3863388 3863388

      References

      Reference overviewApplicationPub Med ID
      Isthmin targets cell-surface GRP78 and triggers apoptosis via induction of mitochondrial dysfunction.
      Chen, M; Zhang, Y; Yu, VC; Chong, YS; Yoshioka, T; Ge, R
      Cell death and differentiation  21  797-810  2014

      Show Abstract
      24464222 24464222
      New blocking antibodies impede adhesion, migration and survival of ovarian cancer cells, highlighting MFGE8 as a potential therapeutic target of human ovarian carcinoma.
      Tibaldi, L; Leyman, S; Nicolas, A; Notebaert, S; Dewulf, M; Ngo, TH; Zuany-Amorim, C; Amzallag, N; Bernard-Pierrot, I; Sastre-Garau, X; Théry, C
      PloS one  8  e72708  2013

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)23977342 23977342
      Dual inhibition of αV integrins and Src kinase activity as a combination therapy strategy for colorectal cancer.
      Jia, J; Starodub, A; Cushman, I; Liu, Y; Marshall, DJ; Hurwitz, HI; Nixon, AB
      Anti-cancer drugs  24  237-50  2013

      Show Abstract
      23275294 23275294
      Synstatin: a selective inhibitor of the syndecan-1-coupled IGF1R-αvβ3 integrin complex in tumorigenesis and angiogenesis.
      Rapraeger, Alan C
      FEBS J., (2013)  2013

      Show Abstract
      23375101 23375101
      Assessment of the integrin alpha5beta1 antagonist JSM6427 in proliferative vitreoretinopathy using in vitro assays and a rabbit model of retinal detachment.
      Zahn, G; Volk, K; Lewis, GP; Vossmeyer, D; Stragies, R; Heier, JS; Daniel, PE; Adamis, AP; Chapin, EA; Fisher, SK; Holz, FG; Löffler, KU; Knolle, J
      Investigative ophthalmology & visual science  51  1028-35  2010

      Show Abstract
      19815730 19815730
      Fluctuations of cytoskeleton-bound microbeads--the effect of bead-receptor binding dynamics.
      C Metzner,C Raupach,C T Mierke,B Fabry
      Journal of physics. Condensed matter : an Institute of Physics journal  22  2010

      Show Abstract
      21386432 21386432
      Endothelial cell lumen and vascular guidance tunnel formation requires MT1-MMP-dependent proteolysis in 3-dimensional collagen matrices.
      Stratman, AN; Saunders, WB; Sacharidou, A; Koh, W; Fisher, KE; Zawieja, DC; Davis, MJ; Davis, GE
      Blood  114  237-47  2009

      Show Abstract Full Text Article
      19339693 19339693
      Integrin alphaVbeta6 is a high-affinity receptor for coxsackievirus A9.
      Heikkilä, O; Susi, P; Stanway, G; Hyypiä, T
      The Journal of general virology  90  197-204  2009

      Show Abstract
      19088289 19088289
      Src, PKCalpha, and PKCdelta are required for alphavbeta3 integrin-mediated metastatic melanoma invasion.
      Putnam, Andrew J, et al.
      Cell Commun. Signal, 7: 10 (2009)  2009

      Show Abstract
      19400942 19400942
      Anti-integrin monoclonal antibody CNTO 95 enhances the therapeutic efficacy of fractionated radiation therapy in vivo.
      Ning, S; Nemeth, JA; Hanson, RL; Forsythe, K; Knox, SJ
      Molecular cancer therapeutics  7  1569-78  2008

      Show Abstract
      18566227 18566227

      Data Sheet

      Title
      MOUSE ANTI-HUMAN INTEGRIN alphaVbeta5 PRESERVATIVE FREE

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      Alternative Format

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies