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MAB1957Z Anti-Integrin β3 Antibody, clone 25E11, azide free

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MAB1957Z
100 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H FC, IP, ICC, FUNC M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB1957Z
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β3 Antibody, clone 25E11, azide free
      Alternate Names
      • CD61
      • MAB1957
      Background InformationThe integrin family of cell adhesion receptors consists of several membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
      Platelet membrane glycoprotein Integrin beta3 or CD61 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP IIb. The GP IIb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets.
      References
      Product Information
      FormatPurified
      PresentationIn buffer of 0.01M phosphate, 0.15M sodium chloride, pH 7.1, containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect Integrin β3 using this Anti-Integrin β3 Antibody, clone 25E11, azide free validated for use in FC, IP & IC.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Immunocytochemistry
      • Affects Function
      Application NotesImmunoprecipitation

      Immunocytochemistry (acetone fixation required).

      Flow cytometry

      Inhibits platelet aggregation, 10 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenNormal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.
      Clone25E11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.

      (original paper claimed IgG2ak)
      IsotypeIgM
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB3
      • GP3A
      • CD61
      • GPIIIa
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 788 amino acids; 87058 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
      PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
      DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
      SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Integrin β3 Antibody, clone 25E11, azide free SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Integrin β3 Antibody, clone 25E11, azide free Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN INTEGRIN b3 (GPIIIa, CD61) -27009762700976
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) PRESERVATIVE FREE MONOCLONAL ANTIBODY - 23787682378768
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) - 35174303517430
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) -26145472614547
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) -26226772622677
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) -27464022746402
      MOUSE ANTI-HUMAN INTEGRIN 3 (GPIIIa, CD61) PRESERVATIVE FREE - 25139662513966

      References

      Reference overviewPub Med ID
      Neutralization of malaria GPI in vitro by serum IgG from malaria exposed individuals.
      de Souza JB, Runglall M, Corran PH, Okell LC, Kumar S, Gowda DC, Couper KN, Riley EM
      Infect Immun  2010

      Show Abstract
      20566691 20566691
      Integrin alphavbeta1 promotes infection by human metapneumovirus.
      Cseke, G; Maginnis, MS; Cox, RG; Tollefson, SJ; Podsiad, AB; Wright, DW; Dermody, TS; Williams, JV
      Proceedings of the National Academy of Sciences of the United States of America  106  1566-71  2009

      Show Abstract Full Text Article
      19164533 19164533
      Differential regulation of beta1 integrins by chemoattractants regulates neutrophil migration through fibrin.
      Loike, JD; Cao, L; Budhu, S; Marcantonio, EE; El Khoury, J; Hoffman, S; Yednock, TA; Silverstein, SC
      The Journal of cell biology  144  1047-56  1999

      Show Abstract
      10085300 10085300
      Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer.
      Siitonen, S M, et al.
      Am. J. Pathol., 142: 1081-9 (1993)  1993

      Show Abstract
      7682759 7682759

      Data Sheet

      Title
      MOUSE ANTI-HUMAN INTEGRIN β3 (GPIIIa, CD61) PRESERVATIVE FREE MONOCLONAL ANTIBODY

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      Alternative Format

      Catalog Number Description  
      MAB1957 Anti-Integrin β3 Antibody, clone 25E11 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies