Product Information
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
ImmunogenHuman
Clone14A231
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Monkey
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-IKKα Mouse mAb (14A231) SDS

Title

Safety Data Sheet (SDS) 

Anti-IKKα Mouse mAb (14A231) Certificates of Analysis

TitleLot Number
OP133

References

Reference overview
Zandi, E., et al. 1998 Science 281, 1360.
Didonato, J.A., et al. 1997 Nature 388, 548.
Lee, F.S., et al. 1997 Cell 88, 213.
Mercurio, F., et al. 1997 Science 278, 860.
Regnier, C.H., et al. M. 1997 Cell 90, 373.
Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
Application Data
Detection of human IKKα by immunoblotting. Sample: Whole cell lysate (30 µg) from Daudi cells. Primary antibody: Anti-IKKα Mouse mAb (14A231) (Cat. No. OP133) (1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.
BackgroundNF-κB (nuclear factor κB) is sequestered in the cytoplasm by IκB family of inhibitory proteins that mask the nuclear localization signal of NF-κB thereby preventing translocation of NF-κB to the nucleus. External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IκB releasing NF-κB dimers. NF-κB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IκBα is autoregulated. IκB proteins are phosphorylated by IκB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β and IKK3/γ. In vitro, IKK1/α and IKK2/β can form homo- and heterodimers that can phosphorylate IκBs at the regulatory serine residues directly. IKK1/α and IKK2/β are phosphorylated by NF-κB-inducing kinase (NIK) and MAP kinase kinase kinase-1 (MEKK1), respectively. Targeted disruption of IKK1/α gene in mice results in skin and limb abnormalities and death of newborns.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of full-length human IKKα/IKK1 fused to a His•Tag® sequence
Clone14A231
IsotypeIgG₁
Specieshuman, monkey, mouse
Positive controlDaudi cells
FormLiquid
FormulationIn PBS, 0.2% gelatin, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesZandi, E., et al. 1998 Science 281, 1360.
Didonato, J.A., et al. 1997 Nature 388, 548.
Lee, F.S., et al. 1997 Cell 88, 213.
Mercurio, F., et al. 1997 Science 278, 860.
Regnier, C.H., et al. M. 1997 Cell 90, 373.
Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA 94, 11758.
Verma, I.M., et al. 1995 Genes. Dev. 9, 2723.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies