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MABE232 Anti-IGF2BP2 Antibody, clone 5E10-3E1

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MABE232
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE232
      DescriptionAnti-IGF2BP2 Antibody, clone 5E10-3E1
      Alternate Names
      • Insulin-like growth factor 2 mRNA-binding protein 2
      • IGF2 mRNA-binding protein 2
      • IMP-2
      • Hepatocellular carcinoma autoantigen p62
      • IGF-II mRNA-binding protein 2
      • VICKZ family member 2
      Background InformationInsulin-like growth factor 2 mRNA-binding protein 2 (IGF2BP2) is a member of the IGF-II mRNA binding protein family that posttranscriptionally regulates IGF2 during developmental stages. IGF2 mRNA-binding protein family members (IGF2BP1, 2 and 3) bind UTR region of IGF2 transcripts and are implicated in the transport of RNA targets to enable protein synthesis at specific locations in the cytoplasm. Studies have indicated variations in the IGF2BP2 gene may affect fetal glucose metabolism and plays a role in type 2 diabetes. In addition to playing a role in glucose metabolism and dieabetes, the insulin-like growth factor (IGF) signaling axis plays an important role in cancer biology. Research has shown IGF2 to be emerging as an important gene for ovarian cancer.
      References
      Product Information
      FormatPurified
      Control
      • HepG2 cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-IGF2BP2 Antibody, clone 5E10-3E1 (mouse monoclonal antibody) validated in WB to detect IGF2BP2 also known as Insulin-like growth factor 2 mRNA-binding protein 2, IGF2 mRNA-binding protein 2, IMP-2, Hepatocellular carcinoma autoantigen p62.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHistidine-tagged recombinant protein corresponding to human IGF2BP2.
      Clone5E10-3E1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains several four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
      Gene Symbol
      • IGF2BP2
      • IMP2
      • VICKZ2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.

      SUBUNIT STRUCTURE: Interacts with HNRPD and IGF2BP1.

      SUBCELLULAR LOCATION: Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.

      TISSUE SPECIFICITY: Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.

      DOMAIN: The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.

      MISCELLANEOUS: Autoantibodies against IGF2BP2 are detected in sera from some patients with hepatocellular carcinoma.

      SEQUENCE SIMILARITIES: Belongs to the RRM IMP/VICKZ family.

      Contains 4 KH domains.

      Contains 2 RRM (RNA recognition motif) domains.

      SEQUENCE CAUTION: The sequence AAH21290.1 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight~66 kDa observed. An uncharacterized band at ~50 kDa may be observed in some lysates. Alternative splicing can result in 5 isoforms with molecular weights ranging from 55-66 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HepG2 cell lysate.

      Western Blot Analysis: A 1:20,000 dilution of this antibody detected IGF2BP2 on 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-IGF2BP2 Antibody, clone 5E10-3E1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-IGF2BP2 Antibody, clone 5E10-3E1 Certificates of Analysis

      TitleLot Number
      Anti-IGF2BP2, clone 5E10-3E1 - 2287213 2287213
      Anti-IGF2BP2, clone 5E10-3E1 - 2377083 2377083
      Anti-IGF2BP2, clone 5E10-3E1 - 3787646 3787646
      Anti-IGF2BP2, clone 5E10-3E1 - NRG1941034 NRG1941034
      Anti-IGF2BP2, clone 5E10-3E1 -2767709 2767709
      Anti-IGF2BP2, clone 5E10-3E1 -2892868 2892868
      Anti-IGF2BP2, clone 5E10-3E1 Monoclonal Antibody 3081973

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies