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AB1284 Anti-Heme Oxygenase 1 Antibody

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50 µg  
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      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberAB1284
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Heme Oxygenase 1 Antibody
      Alternate Names
      • HO-1
      • HSP32
      Product Information
      FormatAffinity Purified
      • Lung
      PresentationAffinity Purified immunoglobulin. Liquid in PBS containing 0.01M sodium azide.
      Quality LevelMQ100
      ApplicationThis Anti-Heme Oxygenase 1 Antibody is validated for use in IH, WB for the detection of Heme Oxygenase 1.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern Blot: 1:1,000 (using ECL). A single band of ~32 kD is observed. Reactivity can be completely abolished by pre-adsorption of the antibody with the complementary peptide (Catalog Number AG253). Also available are microsomal tissue preparations for control purposes (Rat spleen microsomal preparation, Catalog Number AG257).

      Immunohistochemistry: 1:50-1:1,000 on human airway biopsy, human spleen, human kidney and porcine arteries.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenA synthetic peptide with the amino acid sequence H-D-L-S-E-A-L-K-E-A-T-K-E-V-H-C-NH2 corresponding to amino acid residues [12-25] + Cys-NH2 of the human HO-1 protein (Yoshida et al., 1988).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes Heme oxygenase-1.
      Species Reactivity
      • Bovine
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHeme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
      Gene Symbol
      • HMOX1
      • HO
      • OTTHUMP00000028925
      • HO1
      • HO-1
      • bK286B10
      Non-Reactive Species
      • Canine
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P09601 # Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed.
      SIZE: 288 amino acids; 32819 Da
      SIMILARITY: SwissProt: P09601 ## Belongs to the heme oxygenase family.
      Molecular Weight32 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information


      Anti-Heme Oxygenase 1 Antibody SDS


      Safety Data Sheet (SDS) 

      Anti-Heme Oxygenase 1 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 2855527 2855527
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3288524 3288524
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3417922 3417922
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3466322 3466322
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3521089 3521089
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3674884 3674884
      RABBIT ANTI-HEME OXYGENASE-1 (HO-1; HEAT SHOCK PROTEIN 32) - 3702336 3702336


      Reference overviewPub Med ID
      In vivo siRNA delivery of Keap1 modulates death and survival signaling pathways and attenuates concanavalin-A-induced acute liver injury in mice.
      González-Rodríguez, Á; Reibert, B; Amann, T; Constien, R; Rondinone, CM; Valverde, ÁM
      Disease models & mechanisms  7  1093-100  2014

      Show Abstract
      24997191 24997191
      Protein tyrosine phosphatase 1B modulates GSK3β/Nrf2 and IGFIR signaling pathways in acetaminophen-induced hepatotoxicity.
      Mobasher, MA; González-Rodriguez, A; Santamaría, B; Ramos, S; Martín, MÁ; Goya, L; Rada, P; Letzig, L; James, LP; Cuadrado, A; Martín-Pérez, J; Simpson, KJ; Muntané, J; Valverde, AM
      Cell death & disease  4  e626  2013

      Show Abstract
      23661004 23661004
      TJ0711, a novel vasodilatory β-blocker, protects SHR rats against hypertension induced renal injury.
      Yang, Juan, et al.
      Am J Transl Res, 5: 279-90 (2013)  2013

      Show Abstract
      23634239 23634239
      Long-lasting neuroprotective effect of sildenafil against 3,4-methylenedioxymethamphetamine- induced 5-hydroxytryptamine deficits in the rat brain.
      Elena Puerta,Lucia Barros-Miñones,Isabel Hervias,Violeta Gomez-Rodriguez,Lourdes Orejana,Neus Pizarro,Rafael de la Torre,Joaquín Jordán,Norberto Aguirre
      Journal of neuroscience research  90  2012

      Show Abstract
      21948520 21948520
      Heme oxygenase-1 could mediate the protective effects of hyperbaric oxygen preconditioning against hepatic ischemia-reperfusion injury in rats.
      Yi Liu,Xue-Jun Sun,Ji Liu,Zhi-Min Kang,Xiao-Ming Deng
      Clinical and experimental pharmacology & physiology  38  2011

      Show Abstract
      21711378 21711378
      Up-regulation of heme oxygenase-1 by isoflurane preconditioning during tolerance against neuronal injury induced by oxygen glucose deprivation.
      Qifang Li, Yesen Zhu, Hong Jiang, Hui Xu, Heping Liu
      Acta biochimica et biophysica Sinica  40  803-10  2008

      Show Abstract
      18776993 18776993
      Resveratrol increases vascular oxidative stress resistance.
      Ungvari, Z; Orosz, Z; Rivera, A; Labinskyy, N; Xiangmin, Z; Olson, S; Podlutsky, A; Csiszar, A
      American journal of physiology. Heart and circulatory physiology  292  H2417-24  2007

      Show Abstract
      17220179 17220179
      Cloning, sequencing and expression of cDNA for chick liver haem oxygenase. Comparison of avian and mammalian cDNAs and deduced proteins
      Evans, C. et al.
      Biochem. J. , 273:659-666 (1991)  1991

      1996964 1996964
      Human heme oxygenase cDNA and induction of its mRNA by hemin.
      Yoshida, T, et al.
      Eur. J. Biochem., 171: 457-61 (1988)  1988

      Show Abstract
      3345742 3345742
      Purification and properties of bovine spleen heme oxygenase. Amino acid composition and sites of action of inhibitors of heme oxidation
      Yoshinaga, T. et al.
      J. Biol. Chem., 257:7778-7785 (1982)  1982

      6806282 6806282