MAB8672-I Anti-HSV1-ICP8 Antibody, clone 10A3

MAB8672-I
100 µL  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      A WB M Purified Monoclonal Antibody
      Description
      Catalogue NumberMAB8672-I
      DescriptionAnti-HSV1-ICP8 Antibody, clone 10A3
      Alternate Names
      • Major DNA-binding protein
      • HSV1-ICP8
      • Infected cell protein 8
      Background InformationMajor DNA-binding protein (UniProt: P04296) is encoded by DBP (also known as Infected cell protein 8, ICP8, UL29) gene (Gene ID: 2703458) in human herpes simplex virus 1. HSV1-ICP8 is a 128 kDa, single stranded DNA binding protein of the herpesviridae family. It is an essential replication factor for both Herpes Simplex Virus Type 1 (HSV-1) and Adeno-Associated Virus (AAV) DNA replication. It binds tightly and cooperatively to ssDNA and destabilizes dsDNA helices, promotes DNA strand transfer, and renaturation of ssDNA. ICP8 is reported to regulate viral gene expression by repressing transcription from the parental viral genome and stimulating gene expression from the progeny genome. It is known to associate in replication compartments with over 50 cellular and viral proteins-many of which are involved in DNA replication and repair, recombination, and chromatin modeling. In the absence of DNA replication, HSV1-ICP8 is found in the nuclear framework-associated structures (pre-replicative sites); however, as viral DNA replication proceeds, it migrates to globular intranuclear structures (replication compartments). (Ref.: Glauser, D.L., et al. (2007). J. Virol. 81(9): 4732-4743; Tolun, G., et al. (2013). Nucleic Acids Res. 41(11): 5927 5937).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis mouse monoclonal Anti-HSV1-ICP8, clone 10A3 , Cat. No. MAB8672-I is validated for use in Western Blotting for the detection of Herpes Simplex Virus Infected cell protein 8.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected HSV1-ICP8 in HeLa cells (Glauser, D.L., et. al. (2007). J Virol. 81(9):4732-43).
      Biological Information
      ImmunogenICP8 purified from U-35-VERO cells.
      Clone10A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10A3 detects ICP-8 protein in human herpes simplex virus 1.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • DBP
      • ICP8
      • UL29
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~128 kDa observed; 128.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in using recombinant protein.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected 1.25 µg of 1CB8 recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications