400064 Anti-Glucose Transporter-4 Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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400064-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewRecognizes the ~40-43 kDa GLUT-4 protein.
      Catalogue Number400064
      Brand Family Calbiochem®
      SynonymsAnti-Glut-4
      References
      ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
      James, D.E., et al. 1989. Nature 338, 83.
      James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.1% BSA, pH 7.5.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.5-1 µg/ml)
      Frozen Sections (2-20 µg/ml)
      Immunoblotting (1-10 µg/ml; see comments)
      Immunoprecipitation (1-10 µg/100 µg tissue membrane)
      Application CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
      ImmunogenMouse
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glucose Transporter-4 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glucose Transporter-4 Rabbit pAb Certificates of Analysis

      TitleLot Number
      400064

      References

      Reference overview
      Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
      James, D.E., et al. 1989. Nature 338, 83.
      James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-March-2009 RFH
      SynonymsAnti-Glut-4
      ApplicationELISA (0.5-1 µg/ml)
      Frozen Sections (2-20 µg/ml)
      Immunoblotting (1-10 µg/ml; see comments)
      Immunoprecipitation (1-10 µg/100 µg tissue membrane)
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
      BackgroundGlucose transporter-4 (Glut-4) is a transmembrane protein that facilitates transport of glucose in the cell in an energy-independent fashion. Insulin activates glucose transport in fat cells through a translocation of the insulin-sensitive Glut-4 and by activation of glucose carriers in the plasma membrane. Glut-4 is unique to tissues that exhibit insulin-stimulated glucose uptake (heart and skeletal muscle and adipose tissue).
      HostRabbit
      Immunogen speciesMouse
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
      IsotypeIgG
      Specieshuman, mouse, rat
      FormLiquid
      FormulationIn PBS, 0.1% BSA, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
      James, D.E., et al. 1989. Nature 338, 83.
      James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.