345860 Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10)

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
B, H, M, R R Monoclonal Antibody

Pricing & Availability

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345860-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes GFAP in astrocytes induced by a variety of CNS injuries in human and rat brain tissues.
      Catalogue Number345860
      Brand Family Calbiochem®
      SynonymsAnti-GFAP
      Application Data
      Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
      Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHuman or rat brain tissues
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoprecipitation
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesELISA (0.1-0.5 µg/ml)
      Immunoblotting (0.1-0.5 µg/ml)
      Frozen Sections (10-50 µg/ml)
      Immunoprecipitation (2-5 µg/ml)
      Paraffin Sections (10-50 µg/ml, see comments)
      Application CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenbovine GFAP
      ImmunogenBovine Brain
      Clone2.2B10
      HostRat
      IsotypeIgG2a
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) Certificates of Analysis

      TitleLot Number
      345860

      References

      Reference overview
      Tohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
      Lee, V.M., et al. 1984. J. Neurochem. 42, 25.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-August-2007 RFH
      SynonymsAnti-GFAP
      ApplicationELISA (0.1-0.5 µg/ml)
      Immunoblotting (0.1-0.5 µg/ml)
      Frozen Sections (10-50 µg/ml)
      Immunoprecipitation (2-5 µg/ml)
      Paraffin Sections (10-50 µg/ml, see comments)
      Application Data
      Detection of human glial fibrillary acidic protein by immunohistochemistry. Sample: Astrocytoma tissue. Primary antibody: Anti-Glial Fibrillary Acidic Protein Rat mAb (2.2B10) (Cat. No. 345860) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified rat monoclonal antibody. Recognizes the ~55 kDa glial fibrillary acidic protein (GFAP).
      HostRat
      Immunogen speciesBovine Brain
      Immunogenbovine GFAP
      Clone2.2B10
      IsotypeIgG2a
      Speciesbovine, human, rodent
      Positive controlHuman or rat brain tissues
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsProduct is not to be used for animal treatment, in vivo research, or in any other contact procedure with livestock. Stains reactive rodent and human brain astrocytes induced by a variety of central nervous system injuries. This antibody is suitable for immunohistochemical staining of Bouin's-fixed, frozen, or paraffin-embedded tissue sections. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesTohyama, T., et al. 1993. Am. J. Pathol. 142, 871.
      Lee, V.M., et al. 1984. J. Neurochem. 42, 25.