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MAB10404 Anti-Ghrelin Antibody, clone 1ML-1D7

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      Key Specifications Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICCMAscitesMonoclonal Antibody
      Catalogue NumberMAB10404
      DescriptionAnti-Ghrelin Antibody, clone 1ML-1D7
      Alternate Names
      • ghrelin/obestatin prepropeptide1
      • ghrelin/obestatin2
      • ghrelin, growth hormone secretagogue receptor ligand1
      • obestatin1
      • ghrelin/obestatin preprohormone
      • Motilin-related peptide2
      • ghrelin1
      • M46 protein3
      • Growth hormone secretagogue
      Background InformationGhrelin is a 28 amino acids peptide with an essential n-octonayl modification at Serine 3. This peptide hormone is known as an endogenous ligand for the growth hormone secretagogue receptor and shares sequence similarity with the peptide hormone Motilin. Ghrelin displays pleotropic actions including growth hormone secretion, feeding behaviour and gastrointestinal motility. It is predominantly produced by enteroendocrine cells from the gastrointestinal tract, but has been shown to play a significant role in neurotrophy, particularly in the hippocampus, and appears to be essential for cognitive adaptation to changing environments and the process of learning.
      Product Information
      • Mouse stomach tissue lysate
      PresentationUnpurified mouse monoclonal ascites with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationDetect Ghrelin using this Anti-Ghrelin Antibody, clone 1ML-1D7 validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a previous lot detected Ghrelin in A431 cells.
      Biological Information
      ImmunogenLinear Peptide corresponding to the N-terminus of human Ghrelin.
      Cloneclone 1ML-1D7
      SpecificityThis antibody recognizes Ghrelin at the N-terminus.
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq].
      Gene Symbol
      • MTLRP1
      • GHRL
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility By similarity.


      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.

      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.

      Amidation of Leu-98 is essential for obestatin activity by similarity.

      SEQUENCE SIMILARITIES: Belongs to the motilin family.

      MASS SPECTROMETRY: Molecular mass is 3398.9±0.3 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10, O-decanoylated form.

      Molecular mass is 3397.2±0.5 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C10:1, O-decenoylated form.

      Molecular mass is 3371.3±0.1 Da from positions 24 - 51. Determined by ESI. Ghrelin-28-C8, O-octanoylated form.

      Molecular mass is 3243.6±0.4 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C10, O-decanoylated form.

      Molecular mass is 3214.6±0.6 Da from positions 24 - 50. Determined by ESI. Ghrelin-27-C8, O-octanoylated form.
      Molecular Weight13 kDa calculated and observed
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse stomach tissue lysate.

      Western Blot Analysis: A 1:1,000 dilution of this antibody detected Ghrelin in 10 µg of mouse stomach tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information


      Anti-Ghrelin Antibody, clone 1ML-1D7 SDS


      Safety Data Sheet (SDS) 

      Anti-Ghrelin Antibody, clone 1ML-1D7 Certificates of Analysis

      TitleLot Number
      Anti-Ghrelin, clone 1ML-1D7 - 2466956 2466956
      Anti-Ghrelin, clone 1ML-1D7 - 2016003 2016003
      Anti-Ghrelin, clone 1ML-1D7 - 3167065 3167065
      Anti-Ghrelin, clone 1ML-1D7 - 3492237 3492237
      Anti-Ghrelin, clone 1ML-1D7 - 3885500 3885500
      Anti-Ghrelin, clone 1ML-1D7 - 4004375 4004375
      Anti-Ghrelin, clone 1ML-1D7 - NRG1719642 NRG1719642
      Anti-Ghrelin, clone 1ML-1D7 -2529392 2529392
      Anti-Ghrelin, clone 1ML-1D7 -2639906 2639906
      Anti-Ghrelin, clone 1ML-1D7 -2640538 2640538


      Reference overviewPub Med ID
      Histopathologic and immunohistochemical sequelae of bariatric embolization in a porcine model.
      Paxton, BE; Alley, CL; Crow, JH; Burchette, J; Weiss, CR; Kraitchman, DL; Arepally, A; Kim, CY
      Journal of vascular and interventional radiology : JVIR  25  455-61  2014

      Show Abstract
      24462005 24462005

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      Life Science Research > Antibodies and Assays > Primary Antibodies