PK1111 Anti-GSK-3β Rabbit pAb

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Pricing & Availability

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PK1111-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~46 kDa GSK-3β protein in mouse small intestine tissue.
      Catalogue NumberPK1111
      Brand Family Calbiochem®
      Application Data
      Detection of mouse GSK-3β by immunoblotting. Sample: Whole tissue extract from mouse small intestine. Primary antibody: Anti-GSK-3β Rabbit pAb (Cat. No. PK1111). Detection: chemiluminescence.
      References
      ReferencesGarrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
      Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.
      Product Information
      FormLiquid
      FormulationIn PBS, 30% glycerol, 0.5% BSA.
      Positive controlMouse small intestine
      Preservative0.01% thimerosal
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (0.5-4 µg/ml)
      Immunohistochemistry (10-30 µg/ml; see comments)
      Application CommentsThis antibody has been reported to work for immunohistochemistry in the range of 10-30 µg/ml, but the optimal concentration should be determined for each experiment. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human GSK-3β
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-GSK-3β Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GSK-3β Rabbit pAb Certificates of Analysis

      TitleLot Number
      PK1111

      References

      Reference overview
      Garrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
      Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2016 JSW
      ApplicationImmunoblotting (0.5-4 µg/ml)
      Immunohistochemistry (10-30 µg/ml; see comments)
      Application Data
      Detection of mouse GSK-3β by immunoblotting. Sample: Whole tissue extract from mouse small intestine. Primary antibody: Anti-GSK-3β Rabbit pAb (Cat. No. PK1111). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~46 kDa GSK-3 protein.
      BackgroundGlycogen synthase kinase 3 (GSK-3) is a serine/threonine protein kinase that plays a role in the regulation of cell survival and in the Wnt signaling pathway. Its activity can be inhibited by Akt-mediated phosphorylation at Ser21 in GSK-3α and Ser9 in GSK-3β. GSK-3 is also known to phosphorylate the six isoforms of Tau, indicating a link to Alzheimer's disease.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant human GSK-3β
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlMouse small intestine
      FormLiquid
      FormulationIn PBS, 30% glycerol, 0.5% BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative0.01% thimerosal
      CommentsThis antibody has been reported to work for immunohistochemistry in the range of 10-30 µg/ml, but the optimal concentration should be determined for each experiment. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesGarrido, J.J., et al. 2007.FEBS Lett. 8, 1579.
      Meares, G.P. and Jope, R.S. 2007.J Biochem. 23, 16989.