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MABT1363 Anti-GM130 Antibody, clone 4A3

MABT1363
200 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1363
      DescriptionAnti-GM130 Antibody, clone 4A3
      Alternate Names
      • Golgin subfamily A member 2
      • 130 kDa cis-Golgi matrix protein
      Background InformationGolgin subfamily A member 2 (UniProt: Q62839; also known as 130 kDa cis-Golgi matrix protein, GM130) is encoded by the Golga2 gene (Gene ID: 64528) in rat. GM130 is a peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton to maintain the structure of the Golgi apparatus, and as a vesicle tether that facilitates vesicle fusion to the Golgi membrane. GM130 is a homotetramer with a flexible rod-like structure. It has a nuclear localization sequence (aa 26-49) that mediates interaction with importin-alpha and recruits it to the Golgi membrane and liberates TPX2. GM130 promotes the mitotic spindle pole assembly by activating TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle. Phosphorylation of GM130 at Ser37 by CDK1 at the onset of mitosis inhibits its interaction with p115/USO1, which prevents its tethering of COPI vesicles and thereby inhibits transport through the Golgi apparatus during mitosis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GM130, clone 4A3, Cat. No. MABT1363, is a mouse monoclonal antibody that detects Golgin subfamily A member 2. (GM130) and is tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human liver cancer tissue.

      Western Blotting Analysis: A representative lot detected GM130 in Western Blotting applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).

      Immunocytochemistry Analysis: A representative lot detected GM130 in Immunocytochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51; Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12).

      Immunohistochemistry Analysis: A representative lot detected GM130 in Immunohistochemistry applications (Seemann, J., et. al. (2002). Science. 295(5556):848-51).

      Immunoprecipitation Analysis: A representative lot detected GM130 in Immunoprecipitation applications (Diao, A., et. al. (2003) J Cell Biol. 160(2):201-12; Diao, A., et. al. (2008) J Cell Biol. 283(11):6957-67).
      Biological Information
      ImmunogenA full length recombinant human Golgin subfamily A member 2 (GM130).
      Clone4A3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4A3 detected Golgin subfamily A member 2 (GM130) in human liver cancer and human intestine sections.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Golga2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight112.87 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human small intestine tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected GM130 in human small intestine tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

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